Additional Information
| Product Name: | Anti-GAPDH |
|---|---|
| Also Known As: | Anti-GAPDH (GA1R) Loading Control Mouse Monoclonal Antibody |
| Catalog No.: | Y1041 |
| Size: | 100 µg |
| Molecular Weight: | ~55/25 kDa |
| Species: | Human |
| Source: | Immunogen: Recombinant GAPDH |
| Stock: | 10 mM PBS (pH 7.2), 10% Glycerol, 0.09% NaN3 (sodium azide) |
| Concentration: | 1 µg/µl |
| Quality Assurance: | Guaranteed for detecting endogenous GPADH in 20 µg cell or tissue lysates |
| Storage: | Store at -20°C. Centrifuge after first thaw to maximize product recovery. Aliquot to avoid repeated freeze-thaw cycles. |
| PDF Data Sheet: | PDF Datasheet， MSDS |
| NCBI RefSeq: | N/A |
| Image(s): | (Click image to enlarge)
 Figure 1. Three loading control mAbs reacting against 10 μg/lane of mouse brain tissue lysates. 50 kDa band is Anti-β-Tubulin (BT7R) at 1:2000 dilution (0.5 μg/ml); 42 kDa band is Anti-β-Actin (BA3R) at 1:1000 dilution (1 μg/ml); 37 kDa band is Anti-GAPDH (GA1R) at 1:5000 dilution (0.2 μg/ml)  Figure 2. Left: 1:2000 (0.5μg/mL) Ab dilution used in WB of 5μg/lane tissue lysates from human (1), mouse (2), rat (3), rabbit (4), chicken (5), and hamster (6). Right: WB from BL-21 bacteria (1), Sf9 insect (2), and Saccharomyces cerevisiae (3) |
| Shipping Method: | Wet ice shipping |
| References: | N/A |
Details
Specificity
Recognizes native and denatured forms of GAPDH (~37kDa)
Isotype
Mouse IgG1
Clone
GA1R
Purification
Protein A affinity chromatography from mouse ascites fluid
Applications
Dot, ELISA, IS, WB
Cross Reactivity
GAPDH from BL-21 bacteria, Sf9 insect, Saccharomyces cerevisiae (yeast), human, mouse, rat, rabbit, chicken, and hamster. GAPDH from other species may also be detectable
Working Conditions
WB (with ECL): 1:1000-100,000 dilution
For best results with other assays (e.g.: Dot, ELISA, IS, etc), please determine optimal working dilution by titration test
Images
(Click image to enlarge)
Figure 1. Three loading control mAbs reacting against 10 μg/lane of mouse brain tissue lysates. 50 kDa band is Anti-β-Tubulin (BT7R) at 1:2000 dilution (0.5 μg/ml); 42 kDa band is Anti-β-Actin (BA3R) at 1:1000 dilution (1 μg/ml); 37 kDa band is Anti-GAPDH (GA1R) at 1:5000 dilution (0.2 μg/ml)
Figure 2. Left: 1:2000 (0.5μg/mL) Ab dilution used in WB of 5μg/lane tissue lysates from human (1), mouse (2), rat (3), rabbit (4), chicken (5), and hamster (6). Right: WB from BL-21 bacteria (1), Sf9 insect (2), and Saccharomyces cerevisiae (3)
Figure 1. Three loading control mAbs reacting against 10 μg/lane of mouse brain tissue lysates. 50 kDa band is Anti-β-Tubulin (BT7R) at 1:2000 dilution (0.5 μg/ml); 42 kDa band is Anti-β-Actin (BA3R) at 1:1000 dilution (1 μg/ml); 37 kDa band is Anti-GAPDH (GA1R) at 1:5000 dilution (0.2 μg/ml)
Figure 2. Left: 1:2000 (0.5μg/mL) Ab dilution used in WB of 5μg/lane tissue lysates from human (1), mouse (2), rat (3), rabbit (4), chicken (5), and hamster (6). Right: WB from BL-21 bacteria (1), Sf9 insect (2), and Saccharomyces cerevisiae (3)
