Additional Information
| Product Name: | Anti-6xHis |
|---|---|
| Also Known As: | Anti-His (HIS.H8) Mouse Monoclonal Antibody |
| Catalog No.: | Y1011 |
| Size: | 100 µg |
| Molecular Weight: | ~55/25 kDa |
| Species: | N/A |
| Source: | Immunogen: HHHHHH (6x His) synthetic peptide conjugated to KLH |
| Stock: | 10 mM PBS (pH 7.2), 10% Glycerol, 0.09% NaN3 (sodium azide) |
| Concentration: | 1 µg/µl |
| Quality Assurance: | WB (with ECL): 1-2 ng of His-tagged fusion proteins |
| Storage: | Store at -20°C. Centrifuge after first thaw to maximize product recovery. Aliquot to avoid repeated freeze-thaw cycles. |
| PDF Data Sheet: | PDF Datasheet， MSDS |
| NCBI RefSeq: | N/A |
| Image(s): | (Click image to enlarge)
 Figure 1. Left: untransfected control; Right: anti-His (in red) on His-tagged fusion proteins in HEK293 cells. Both counterstained with DAPI (in blue)  Figure 2. Comparison between anti-His tag (HIS.H8) mAb with two different vendor Abs, probed against a standard ladder (Qiagen Cat. No. 34705) containing five different His-tagged proteins. All Ab dilutions are 1:2000 (0.5 μg/ml)  Figure 3. Standard ladder containing five different His-tagged proteins; untransfected control (Lane 1), HEK293 cells transfected with His-tagged protein vector (Lane 2) |
| Shipping Method: | Wet ice shipping |
| References: | N/A |
Details
Specificity
Recognizes the epitope of 6xHis-tags encoded by many commercially available vectors, regardless of the tag's location in the fusion protein sequences (i.e.: reacts with N-terminal, C-terminal or internal 6xHis-tags)
Isotype
Mouse IgG2b
Clone
HIS.H8
Purification
Protein A affinity chromatography from mouse ascites fluid
Applications
Dot, ELISA, IP, IS, WB
Working Conditions
WB (with ECL): 1:1000-3000
IS: 1:500-2000 dilution
For best results with other assays (e.g.: Dot, ELISA, IP, etc), please determine optimal working dilution by titration test
Images
(Click image to enlarge)
Figure 1. Left: untransfected control; Right: anti-His (in red) on His-tagged fusion proteins in HEK293 cells. Both counterstained with DAPI (in blue)
Figure 2. Comparison between anti-His tag (HIS.H8) mAb with two different vendor Abs, probed against a standard ladder (Qiagen Cat. No. 34705) containing five different His-tagged proteins. All Ab dilutions are 1:2000 (0.5 μg/ml)
Figure 3. Standard ladder containing five different His-tagged proteins; untransfected control (Lane 1), HEK293 cells transfected with His-tagged protein vector (Lane 2)
Figure 1. Left: untransfected control; Right: anti-His (in red) on His-tagged fusion proteins in HEK293 cells. Both counterstained with DAPI (in blue)
Figure 2. Comparison between anti-His tag (HIS.H8) mAb with two different vendor Abs, probed against a standard ladder (Qiagen Cat. No. 34705) containing five different His-tagged proteins. All Ab dilutions are 1:2000 (0.5 μg/ml)
Figure 3. Standard ladder containing five different His-tagged proteins; untransfected control (Lane 1), HEK293 cells transfected with His-tagged protein vector (Lane 2)
