Additional Information
| Product Name: | PolySUMO Capture & Release Kit |
|---|---|
| Also Known As: | N/A |
| Catalog No.: | J4410 |
| Size: | 1 Kit |
| Molecular Weight: | N/A |
| Species: | N/A |
| Source: | N/A |
| Stock: | Enzyme stocked in 20 mM Tris, 150 mM NaCl, 2 mM βME, 10% Glycerol |
| Concentration: | See tube label |
| Quality Assurance: | Enzyme >95% by SDS-PAGE |
| Storage: | Store at -80°C; avoid multiple freeze-thaw cycles |
| PDF Data Sheet: | PDF datasheet, MSDS |
| NCBI RefSeq: | N/A |
| Image(s): | No |
| Shipping Method: | Dry ice shipping |
| References: | Bruderer R, et al. Purification and identification of endogenous polySUMO conjugates. (2011) EMBO Rep. 12(2):142-8. |
Details
This kit is sufficient for purification of polysumoylated proteins from 100 mg or 20 X 5 mg cell or tissue extracts at a concentration of 5 mg/ml.
Description
The PolySUMO Capture & Release Kit is designed to rapidly enrich polysumoylated proteins from cell or tissue extracts within 6 hours according to a published method (reference 1) with modifications. This approach uses the SIM domains of RNF4 to capture polysumoylated proteins, which are subsequent released by incubating with a competitive peptide that binds SIM.
Components
• GST-4XSIM (4 mg/ml)..............................................1 ml
• GST (4 mg/ml).......................................................1.2 ml
• 2X Elution peptide ............................................ 0.45 ml
• Glutathione Agarose Resin(50% slurry)......... 1.5 ml
• Iodoacetamine (IAA)............................................25 mg
• 20X Cell Lysis Buffer..................................... 2 X 1.0 ml
• 1 M NaCl .......................................................4 X 1.25 ml
Images
(Click image to enlarge)
1. Cell lysates before pulldown
2. Cell lysates after pulldown
3. Elution
