Additional Information
| Product Name: | ML323 |
|---|---|
| Also Known As: | N-(4-(1H-1,2,3-triazol-1-yl)benzyl)-2-(2-isopropylphenyl)-5-methylpyrimidin-4-amine |
| Catalog No.: | F4121 |
| Size: | 25 mg |
| Molecular Weight: | 384.48 Da |
| Species: | N/A |
| Source: | Synthetic |
| Stock: | Powder |
| Concentration: | N/A |
| Quality Assurance: | >99% by HPLC and NMR |
| Storage: | Eligible for room temperature shipping. Store at -20°C upon receiving; protect from air and light. |
| PDF Data Sheet: | PDF Datasheet, MSDS |
| NCBI RefSeq: | N/A |
| Image(s): | (Click image to enlarge) Purity check of ML323 by HPLC |
| Shipping Method: | Room temperature shipping |
| References: | Liang Q, et al. (2014) Nat Chem Biol. 10(4):298-304. |
Details
ML323 is a cell permealbe, selective and highly potent inhibitor of USP1-UAF1 with IC₅₀ of 76 nM in a Ub-Rho assay and 174 nM and 820 nM in orthogonal gel-based assays using K63-linked diubiquitin (di-Ub) and monoubiquitinated PCNA (Ub-PCNA) as substrates, respectively. ML 323 effectively sensitized cisplatin-resistant NSCLC H596 cells and U2OS osteosarcoma cells to cisplatin since it simultaneously targets two major DNA damage response pathways (TLS and FA) by inhibiting USP1-UAF1.
How to use
ML323 was used at 10-30 µM in various in vitro assay. Images
(Click image to enlarge)
Purity check of ML323 by HPLC
ML323 was used at 10-30 µM in various in vitro assay. Images
(Click image to enlarge)
Purity check of ML323 by HPLC
