Product Image(s)
[Cat.# J4310] Rapid 26S Proteasome Purification Kit-S
[Cat.# J4320] Rapid 26S Proteasome Purification Kit-L
| A. Coomassie stained SDS-PAGE of 5 μg purified GST-Ubl. B. Coomassie stained SDS-PAGE of 5 μg purified 6xHis-UIM. C. Coomassie stained SDS-PAGE of 3 μg purified human 26S proteasomes. D. Coomassie stained native-PAGE of 3 μg purified human 26S proteasomes. E. Solution peptidase assay of the purified human 26S proteasome. 150 μl reaction mixtures contained 10 nM purified human 26S proteasome in 50 μM SUC-LLVY-AMC in 20 mM Tris, pH 7.1 at 37°C, 2 mM μME. The released AMC fluorescence was measured using a plate reader with excitation and emission filters at 360/40 and 460/30 nm, respectively. This assay was used to monitor the chymotrypsin-like activity of the 26S proteasome. |
