Additional Information
| Product Name: | Glutathione Xpure Agarose Resin |
|---|---|
| Catalog No.: | P3060-100 |
| Size: | 100 ml |
| Bead (Geometry, size): | Spherical, Standard: ~ 45-165 µm |
| Cross-Linked: | No |
| Ligand: | Glutathione |
| Agarose %: | highly cross-linked 4% Agarose |
| Binding Capacity: | > 10 mg recombinant GST/ml gel (binding capacity may vary for each GST-tagged protein) |
| Volume %: | 50% (v/v) aqueous suspension containing 20% Ethanol |
| Storage Temperature: | 4-8 °C |
| Application: | Batch, FPLC |
| PDF Data Sheet: | Datasheet, MSDS |
| User's Manual: | User’s Guide |
| Image(s): |  |
| Shipping Method: | Wet ice shipping |
Details
Glutathione Agarose Resin consists of glutathione covalently bound to agarose beads. This resin has high binding affinity and specificity to the Glutathione S-transferase (GST) protein. GST-tagged proteins can be highly purified by batch or column purification (low flow rate) in a one step purification method. Bound GST-tagged proteins are easily eluted from the resin with buffers containing reduced glutathione.
GST-E2-25K was purified with Glutathione Agarose Resin using a gravity column.
Lane 1: Molecular weight marker
Lane 2: Cell pellet after sonication
Lane 3: Supernatant after sonication
Lane 4: Flow through after binding
Lane 5: Wash
Lane 6: Elution 1
Lane 7: Elution 2
Lane 8: Elution 3
Lane 9: Elution 4
Lane 10: Elution 5
GST-E2-25K was purified with Glutathione Agarose Resin using a gravity column.
Lane 1: Molecular weight marker
Lane 2: Cell pellet after sonication
Lane 3: Supernatant after sonication
Lane 4: Flow through after binding
Lane 5: Wash
Lane 6: Elution 1
Lane 7: Elution 2
Lane 8: Elution 3
Lane 9: Elution 4
Lane 10: Elution 5
