Additional Information
| Product Name: | Fluorescein labeled K48-Ub2 |
|---|---|
| Also Known As: | Fluorescein K48 Ub2 |
| Catalog No.: | D1110 |
| Size: | 25 µg |
| Molecular Weight: | N/A |
| Species: | Human |
| Source: | Bacterial recombinant |
| Stock: | 20 mM Tris, 150 mM NaCl, 2 mM βME, 10% Glycerol |
| Concentration: | See tube label |
| Quality Assurance: | > 90% by SDS-PAGE, see datasheet for gel image |
| Storage: | Store at -80°C; avoid multiple freeze-thaw cycles |
| PDF Data Sheet: | PDF Datasheet， MSDS |
| NCBI RefSeq: | N/A |
| Image(s): | D1110 image |
| Shipping Method: | Dry ice shipping |
| References: | No |
Details
This product contains an authentic K48-linked isopeptide bond, synthesized by using an enzymatic reaction containing UbE1, E2-25K, Ub(K48R) and Ub-fluorescein. Fluorescein is labeled on the C-terminus of Ub by mutating glycine 76 to a cysteine. In fluorescein labeled K48 Ub2, fluorescein is labeled on the proximal Ub. It is an excellent tool for monitoring deubiquitination that can be visualized by in-gel fluorescent detection of the production of fluorescein labeled monoUb (excitation at ~490 nm, and see product data sheet for examples). The assay is more quantitative and sensitive than immunoblotting of Ub. The detection limit is 10 ng or less.
