Di-ubiquitin Explorer Kit

This kit contains 5 µg each of linear, K6, K11, K27, K29, K33, K48, and K63-linked diubiquitin, a total of eight different diubiquitin forms. It can be used for determining linkage specificity of deubiquitinating enzymes and polyubiquitin-binding proteins. Linear diUb (catalog# D4300) was purified as recombinant protein. K11 diUb (catalog# D3100), K48 diUb (catalog# D1100), and K63 diUb (D2100) were made from recombinant human Ub using specific Ub conjugating enzymes. K6 diUb (catalog# D5110), K27 diUb (catalog# D5210), K29 diUb (catalog# D5310) and K33 diUb (catalog# D5410) were made by chemical synthesis. DiUb from recombinant Ub stained more intense than chemically synthesized ones in Coomassie staining on SDS-PAGE.

Additional Information

Product Name:

Also Known As:

N/A

Catalog No.:

J2000

Size:

1 Kit

Molecular Weight:

N/A

Species:

Human

Source:

Synthetic or bacterial recombinant

Stock:

20 mM Tris, pH7.1 at 37 °C, 150 mM NaCl, 2 mM βME, 10% Glycerol

Concentration:

See tube label

Quality Assurance:

≥90% by SDS-PAGE

Storage:

Store at -80°C. Avoid multiple freeze/thaw cycles.

PDF Data Sheet:

PDF datasheet, MSDS

NCBI RefSeq:

N/A

Image(s):

(Click image to enlarge)

Coomassie-stained SDS-PAGE of 1.5 µg each diUb
1: Linear diUb
2: K6-linked diUb
3: K11-linked diUb
4: K27-linked diUb
5: K29-linked diUb
6: K33-linked diUb
7: K48-linked diUb
8: K63-linked diUb

Shipping Method:

Dry ice shipping

References:

N/A

Details

Description

This kit contains 5 µg each of linear, K6, K11, K27, K29, K33, K48, and K63-linked diubiquitin, a total of eight different diubiquitin forms. It can be used for determining linkage specificity of deubiquitinating enzymes and polyubiquitin-binding proteins. Linear diUb (catalog# D4300) was purified as recombinant protein. K11 diUb (catalog# D3100), K48 diUb (catalog# D1100), and K63 diUb (D2100) were made from recombinant human Ub using specific Ub conjugating enzymes. K6 diUb (catalog# D5110), K27 diUb (catalog# D5210), K29 diUb (catalog# D5310) and K33 diUb (catalog# D5410) were made by chemical synthesis. DiUb from recombinant Ub stained more intense than chemically synthesized ones in Coomassie staining on SDS-PAGE.

Components

• Linear diUb (0.5 mg/ml)...................10 µl
• K6-linked diUb (0.5 mg/ml)...............10 µl
• K11-linked diUb (0.5 mg/ml)..............10 µl
• K27-linked diUb (0.5 mg/ml)..............10 µl
• K29-linked diUb (0.5 mg/ml)..............10 µl
• K33-linked diUb (0.5 mg/ml)..............10 µl
• K48-linked diUb (0.5 mg/ml)..............10 µl
• K63-linked diUb (0.5 mg/ml)..............10 µl

Images:
(Click image to enlarge)

Coomassie-stained SDS-PAGE of 1 µg each diUb
1: Linear diUb
2: K6-linked diUb
3: K11-linked diUb
4: K27-linked diUb
5: K29-linked diUb
6: K33-linked diUb
7: K48-linked diUb
8: K63-linked diUb