Additional Information
| Product Name: | Biotin-Ubiquitin | ||
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| Also Known As: | Biotin-Ub | ||
| Catalog No.: | E1141 | ||
| Size: | 1 mg | ||
| Molecular Weight: | 8.9 kDa | ||
| Species: | Human | ||
| Source: | Synthetic | ||
| Stock: | 20 mM Tris, pH 7.6 at 4 °C, 100 mM NaCl, 10% glycerol | ||
| Concentration: | 1 mg/ml in aqueous buffer | ||
| Quality Assurance: | > 95% by HPLC | ||
| Storage: | Store at -80°C; avoid multiple freeze-thaw cycles | ||
| PDF Data Sheet: | PDF Datasheet， MSDS | ||
| NCBI RefSeq: | N/A | ||
| Image(s): | (Click image to enlarge)
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| Shipping Method: | Dry ice shipping | ||
| References: | N/A |
Details
A single biotin moiety is conjugated on the N-terminal region of ubiquitin. All lysine residues of ubiquitin are still available for formation of polyubiquitin chains. The high binding affinity between biotin and streptavidin allows the use of biotin-ubiquitin for the following in vitro assays: 1) rapid and efficient purification of polyubiquitinated substrate proteins; 2) monitoring substrate ubiquitination using an HRP-conjugated streptavidin antibody; and 3) setting up high throughput TR-FRET assays to monitor substrate or ubiquitin ligase ubiquitination.
Use
Depending on ubiquitination assay conditions, N-terminal biotin-ubiquitin can be mixed with wild type ubiquitin at a combined concentration range of 20-50 μM with a 1:4 to 1:20 ratio of biotin-ubiquitin to wild type ubiquitin. Images: (Click image to enlarge)
Use
Depending on ubiquitination assay conditions, N-terminal biotin-ubiquitin can be mixed with wild type ubiquitin at a combined concentration range of 20-50 μM with a 1:4 to 1:20 ratio of biotin-ubiquitin to wild type ubiquitin. Images: (Click image to enlarge)
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LC-MS of Biotin-Ubiquitin
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