Additional Information
| Product Name: | 6xHis-UbE2C (UbcH10 ) | ||
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| Also Known As: | UBCH10; dJ447F3.2 | ||
| Catalog No.: | C1301 | ||
| Size: | 100 µg | ||
| Molecular Weight: | 19.7 kDa | ||
| Species: | Human | ||
| Source: | Bacterial recombinant | ||
| Stock: | 20 mM Tris, 150 mM NaCl, 2 mM βME, 10% Glycerol | ||
| Concentration: | See tube label | ||
| Quality Assurance: | ~95% by SDS-PAGE, see datasheet for gel image | ||
| Storage: | Store at -80°C; avoid multiple freeze-thaw cycles | ||
| PDF Data Sheet: | PDF Datasheet， MSDS | ||
| NCBI RefSeq: | NM_007019 | ||
| Image(s): | (Click image to enlarge)
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| Shipping Method: | Dry ice shipping | ||
| References: | 1. Hershko A, et al. (1994) J Biol CHem 269, 4940 – 4946. 2. Aristarkhov A, et al. (1996) Proc Natl Acad Sci USA 93(9), 4294 – 4299. 3. Rape M, et al. (2006) Cell. 124(1), 89 – 103. 4. Berlingieri MT, et al. (2007) Oncogene 26, 2136 – 2140. |
Details
UbcH10 (UbE2C) is an E2 enzyme, which is part of the E1, E2, and E3 cascade responsible for ubiquitination of protein substrates. It plays a major role in the mitosis in cell cycle by binding to the anaphase-promoting complex/cyclosome (APC/C), which causes autoubiquitination and degradation of UbcH10. This in turn stabilizes cyclin A, promoting cells enter into S phase. UbcH10 also promotes ubiquitination of many substrates of APC/C.
Images:
(Click image to enlarge)
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Coomassie-stained SDS-PAGE Lane 1: Molecular weight markers Lane 2: 5 µg purified 6xHis-Ube2C (UbcH10 ) |
