6xHis-TEV Protease

Tobacco Etch Virus (TEV) protease is a cysteine protease that is commonly used to remove fusion tags, including His, GST and MBP, at the N or C termini of recombinant proteins. TEV protease specifically recognizes a seven amino acid sequence Glu-Asn-Leu-Tyr-Phe-Gln-Gly/Ser (ENLYFQG/S) and cleaves between Gln and Gly/Ser under a wide range of conditions.

Recombinant 6xHis-TEV was expressed in E. Coli and purified to near homogeneity. A Ser219Asn (S219N) substitution was introduced that has no effect on TEV protease activity, but inhibits auto-cleavage and thus, stabilizing TEV protease during incubation and long-term storage.

We recommend using 3% supplied TEV protease (w/w) to preform reactions at 4 0C for 12-16 h or at 30 0C for 1-4 h. Depending on specific substrates, TEV protease concentration, incubation time, and reaction temperature should be optimized. 6xHis-TEV can be removed by Ni-NTA resin after incubation.

TEV protease maintains high activity under a wide range of temperatures (4 0C - 30 0C), pH (6.0 - 8.5) and buffers, and in the presence of commonly used protease inhibitors.

Additional Information

Product Name:	6xHis-TEV Protease
Also Known As:	TEV protease, NIa, Tobacco etch virus protease
Catalog No.:	P1001-1000
Size:	1 mg
Molecular Weight:	27 kDa
Species:	Tabacco Etch Virus
Source:	Bacterial recombinant
Stock:	100 mM Tris, pH 8.5 at 4 0C, 500 mM NaCl, 5mM DTT, 0.5 mM EDTA and 50% Glycerol
Concentration:	See tube label
Quality Assurance:	> 90% purity based on SDS-PAGE.
Storage:	Store at -80°C; avoid multiple freeze-thaw cycles
PDF Data Sheet:	PDF Datasheet， MSDS
NCBI RefSeq:	N/A
Image(s):
Shipping Method:	Dry ice shipping
References:	1. Lucast LJ.,et al. (2001) Biotechniques 30, 544.

Details

Tobacco Etch Virus (TEV) protease is a cysteine protease that is commonly used to remove fusion tags, including His, GST and MBP, at the N or C termini of recombinant proteins. TEV protease specifically recognizes a seven amino acid sequence Glu-Asn-Leu-Tyr-Phe-Gln-Gly/Ser (ENLYFQG/S) and cleaves between Gln and Gly/Ser under a wide range of conditions. Recombinant 6xHis-TEV was expressed in E. Coli and purified to near homogeneity. A Ser219Asn (S219N) substitution was introduced that has no effect on TEV protease activity, but inhibits auto-cleavage and thus, stabilizing TEV protease during incubation and long-term storage. We recommend using 3% supplied TEV protease (w/w) to preform reactions at 4 0C for 12-16 h or at 30 0C for 1-4 h. Depending on specific substrates, TEV protease concentration, incubation time, and reaction temperature should be optimized. 6xHis-TEV can be removed by Ni-NTA resin after incubation. TEV protease maintains high activity under a wide range of temperatures (4 0C - 30 0C), pH (6.0 - 8.5) and buffers, and in the presence of commonly used protease inhibitors.