3CL Protease Activity Assay Kit I

Additional Information

Product Name:	3CL Protease Activity Assay Kit I
Also Known As:	SARS-CoV-2 3C-like Protease Activity Assay Kit
Catalog No.:	J7510
Size:	1 Kit (120 assays)
Molecular Weight:	N/A
Species:	N/A
Source:	N/A
Stock:	N/A
Concentration:	See tube label
Quality Assurance:	Enzymatic activity validated
Storage:	Store at -80°C; avoid multiple freeze-thaw cycles
PDF Data Sheet:	PDF datasheet, MSDS
NCBI RefSeq:	N/A
Image(s):	J7510 image A. Reactions containing 20µM Dabcyl-KTSAVLQSGFRKME-Edans and 80nM GST-3CLPro. 4µM GST-3CLPro stock was prepared right before the reaction or kept under room temperature for 4 hours prior to addition to reactions. B. Reactions containing 20µM Dabcyl-KTSAVLQSGFRKME-Edans and 80nM GST-3CLPro in the presence of 2% DMSO, 20, 10, 5, 2.5, 1.25, 0.625, 0.3125µM PF-07321332. C. GST-3CLPro activity was measured as shown in B. % of GST-3CLPro activity was plotted vs. Log[uM] of PF-07311332. Data were shown as mean ± SD of three experiments.
Shipping Method:	Dry ice shipping
References:	N/A

Details

The kit can be used for measuring the enzymatic activity of SARS-CoV-2 3CL protease (3CLPro) or screening/profiling 3CLPro inhibitors. Kit I is sufficient for 120 X 50µl assays; Kit II is sufficient for 1,200 X 50µl assays. The kit is optimized for rapidly and quantitatively measuring 3CLPro activity under room temperature.

Key Features: • Optimized for room temperature operation, particularly useful for HTS assays • Rapid and quantitative result within 2-3 hours • Positive 3CLPro inhibitor PF07321332 included • Large excess amount of buffer provided

Components included in the kit

10X GST-3CLPro (Enzyme)................................................... 4µM, 120µl
100X Dabcyl-KTSAVLQSGFRKME-Edans (Substrate)......... 10mM in DMSO, 12µl
100X PF-07321332 (Inhibitor) ................................................ 1.67mM in DMSO, 10µl
5X Assay Buffer....................................................................... 2X1.2ml