Additional Information
| Product Name: | 26S Proteasome-mediated Protein Degradation Kit |
|---|---|
| Also Known As: | N/A |
| Catalog No.: | J4330 |
| Size: | 1 Kit |
| Molecular Weight: | N/A |
| Species: | N/A |
| Source: | N/A |
| Stock: | Enzyme stocked in 20 mM Tris, 150 mM NaCl, 2 mM βME, 10% Glycerol |
| Concentration: | See tube label |
| Quality Assurance: | Enzyme >95% by SDS-PAGE |
| Storage: | Store at -80°C; avoid multiple freeze-thaw cycles |
| PDF Data Sheet: | PDF datasheet, MSD |
| NCBI RefSeq: | N/A |
| Image(s): | N/A |
| Shipping Method: | Dry ice shipping |
| References: | 1. Liu CW, Li X, Thompson D, Wooding K, Chang TL, Tang Z, Yu H, Thomas PJ, DeMartino GN. Mol Cell. 2006;24(1):39-50. 2. Jacobson AD, Zhang NY, Xu P, Han KJ, Noone S, Peng J, Liu CW. J Biol Chem. 2009;284(51):35485-94. 3. Hemantha HP, Bavikar SN, Herman-Bachinsky Y, Haj-Yahya N, Bondalapati S, Ciechanover A, Brik A. J Am Chem Soc. 2014;136(6):2665-73. |
Details
* The supplied materials are sufficient for setting up 32 X 25 ul reactions.
Description
This kit is designed for assaying 26S proteasome-mediated degradation of ubiquitinated proteins in vitro. It contains highly purified active bovine 26S proteasome that has been used by different research groups for assaying protein degradation (see references).
Components
• Bovine 26S proteasome (200 nM).............................100 μl
• Epoxomicin (10 mM in DMSO).....................................3 μl
• ATP (500 mM).............................................................20 μl
• 10X Degradation Buffer.............................................150 μl
Notes
1. 10X Degradation Buffer: 400 mM Tris, pH 7.1 at 37°C, 400 mM NaCl, 50 mM MgCl2, 20 mM β-ME.
2. The supplied proteasome-specific inhibitor epoxomicin was dissolved in DMSO at 10 mM, dilute 100 X for use.
References
1. Liu CW, Li X, Thompson D, Wooding K, Chang TL, Tang Z, Yu H, Thomas PJ, DeMartino GN. Mol Cell. 2006;24(1):39-50.
2. Jacobson AD, Zhang NY, Xu P, Han KJ, Noone S, Peng J, Liu CW. J Biol Chem. 2009;284(51):35485-94.
3. Hemantha HP, Bavikar SN, Herman-Bachinsky Y, Haj-Yahya N, Bondalapati S, Ciechanover A, Brik A. J Am Chem Soc. 2014;136(6):2665-73.
