Additional Information
| Product Name: | 6xHis-Non-cleavable Linear Ub6 | ||
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| Also Known As: | N/A | ||
| Catalog No.: | D4410 | ||
| Size: | 100 µg | ||
| Molecular Weight: | N/A | ||
| Species: | Human | ||
| Source: | Bacterial recombinant | ||
| Stock: | 20 mM Tris, 150 mM NaCl, 2 mM βME, 10% Glycerol | ||
| Concentration: | See tube label | ||
| Quality Assurance: | ~95% by SDS-PAGE, see datasheet for gel image | ||
| Storage: | Store at -80°C; avoid multiple freeze-thaw cycles | ||
| PDF Data Sheet: | PDF Datasheet， MSDS | ||
| NCBI RefSeq: | N/A | ||
| Image(s): | (Click image to enlarge)
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| Shipping Method: | Dry ice shipping | ||
| References: | 1. Hershko A,et al. (1980) Proc Natl Acad Sci USA 77(4), 1783-1786. 2. Pickart CM, (1997) FASEB 11(13), 1055-1066. 3. Hershko A, et al. (1998) Ann Rev Biochem 67, 425-479. 4. Jiang X, et al. (2012) Nature Reviews Immunology 12, 35-48. |
Details
6xHis-non-cleavable linear Ub6 contains a 3xHA tag followed by a TEV cleavage site after the 6xHis tag. The G76V substitution was introduced in the first five Ub moieties and the last Ub has G76. The protein was expressed in E. coli and purified.
Images:
(Click image to enlarge)
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Coomassie-stained SDS-PAGE Lane 1: Molecular weight markers Lane 2: 5 µg purified 6xHis-Non-cleavable Linear Ub6 |
