Sumo 2 (Q90P)

SUMO (small Ub-related modifier) is a Ub-like protein. Three types of SUMO are most commonly studied, SUMO 1, SUMO 2, and SUMO 3. SUMO 2 and SUMO 3 are almost identical isoforms and thus share many functions. Like Ub, SUMO can be conjugated to its target proteins as a polymeric chain. However, SUMO 1 forms chains inefficiently as compared to SUMO 2 and SUMO 3. SUMO is conjugated to target proteins by the E1 (SAE1/SAE2), E2 (Ube2I or Ubc9), E3 (RanBP2/Nup358, amongst others). Protein sumoylation is involved in many cellular processes including gene transcription.

The SUMO2(Q90P) mutant can still form polySUMO2 chains, but these chains are often resistant to desumolyation.

Additional Information

Product Name: Sumo 2 (Q90P)
Also Known As: HSMT3; SMT3B; SUMO3; Smt3A; SMT3H2; MGC117191
Catalog No.: E3230
Size: 500 µg
Molecular Weight: 10.9 kDa
Species: Human
Source: Bacterial recombinant
Stock: 20 mM Tris, 150 mM NaCl, 2 mM βME, 10% Glycerol
Concentration: See tube label
Quality Assurance: ~95% by SDS-PAGE, see datasheet for gel image
Storage: Store at -80°C; avoid multiple freeze-thaw cycles
PDF Data Sheet: PDF DatasheetMSDS
NCBI RefSeq: NM_006937
Image(s): No
Shipping Method: Dry ice shipping
References: 1. Boddy MN, et al. (1996) Oncogene 13, 971 – 982.
2. Bayer P, et al. (1998) J Mol Biol 280, 275 – 286.
3. Melchior F, (2000) Annu Rev Cell Dev Biol 16, 591 – 626.
4. Praefcke GJK, et al. (2012) Trends Biochem Sci 37(1), 23 – 31.
5. Werner A, et al. (2012) Mol Cell 46(3), 287 – 298.

Details

SUMO (small Ub-related modifier) is a Ub-like protein. Three types of SUMO are most commonly studied, SUMO 1, SUMO 2, and SUMO 3. SUMO 2 and SUMO 3 are almost identical isoforms and thus share many functions. Like Ub, SUMO can be conjugated to its target proteins as a polymeric chain. However, SUMO 1 forms chains inefficiently as compared to SUMO 2 and SUMO 3. SUMO is conjugated to target proteins by the E1 (SAE1/SAE2), E2 (Ube2I or Ubc9), E3 (RanBP2/Nup358, amongst others). Protein sumoylation is involved in many cellular processes including gene transcription. The SUMO2(Q90P) mutant can still form polySUMO2 chains, but these chains are often resistant to desumolyation.