6xHis-Nedd8

Nedd8 is an 81 amino acid Ub-like protein. It is conjugated to protein substrates in much the same way as Ub, using the E1, E2, E3 cascade. The specific E1 used is a heterodimer of APPBP1/UBA3. Two E2s have been discovered to follow called Ubc12 and Ube2F. Neddylation causes conformational change of the Cullin protein in the E3 called cullin-RING ligases (CRLs), which enhances the E3 Ub ligase activity of CRLs.

Additional Information

Product Name: 6xHis-Nedd8
Also Known As: NEDD-8, NEDD 8
Catalog No.: E4100
Size: 500 µg
Molecular Weight: 9 kDa
Species: Human
Source: Bacterial recombinant
Stock: 20 mM Tris, 150 mM NaCl, 2 mM βME, 10% Glycerol
Concentration: See tube label
Quality Assurance: ~95% by SDS-PAGE, see datasheet for gel image
Storage: Store at -80°C; avoid multiple freeze-thaw cycles
PDF Data Sheet: PDF DatasheetMSDS
NCBI RefSeq: NM_006156
Image(s): No
Shipping Method: FedEx
References: 1. Kumar S, et al. (1993) Biochem Biophys Res Commun 195(1), 393 – 399.
2. Whitby FG, et al. (1998) J Biol Chem 273(52), 34983 – 34991.
3. Soucy TA, et al. (2010) Genes Cancer 1(7), 708 – 7016.

Details

Nedd8 is an 81 amino acid Ub-like protein. It is conjugated to protein substrates in much the same way as Ub, using the E1, E2, E3 cascade. The specific E1 used is a heterodimer of APPBP1/UBA3. Two E2s have been discovered to follow called Ubc12 and Ube2F. Neddylation causes conformational change of the Cullin protein in the E3 called cullin-RING ligases (CRLs), which enhances the E3 Ub ligase activity of CRLs.