Additional Information
Product Name: | Linear Polyubiquitin Chain Capture Kit |
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Also Known As: | N/A |
Catalog No.: | J4450 |
Size: | 1 Kit |
Molecular Weight: | N/A |
Species: | N/A |
Source: | N/A |
Stock: | Enzyme stocked in 20 mM Tris, 150 mM NaCl, 2 mM βME, 10% Glycerol |
Concentration: | See tube label |
Quality Assurance: | Enzyme >95% by SDS-PAGE |
Storage: | Store at -80°C; avoid multiple freeze-thaw cycles |
PDF Data Sheet: | PDF datasheet, MSDS |
NCBI RefSeq: | N/A |
Image(s): | (Click image to enlarge) |
Shipping Method: | Dry ice shipping |
References: | N/A |
Details
Description
This kit is designed for enrichment of cellular proteins conjugated with linear polyubiquitin chains using GST-NEMO (UBAN). Polyubiquitinated proteins bound on GST-NEMO (UBAN) can be precipitated using glutathione resin and eluted by a buffer containing 10 mM glutathione. Polyubiquitinated proteins can be assayed by immunoblotting or mass spectrometry.
Components
• GST-NEMO (UBAN) (4 mg/ml)..................................0.5 ml
• GST (4 mg/ml)...........................................................0.5 ml
• Iodoacetamide (IAA)..................................................25 mg
• Glutathione (reduced)...............................................100 mg
• Glutathione Resin (50% slurry).................................1.5 ml
Images:
(Click image to enlarge)