Polyubiquitinated E2-25K

Polyubiquitinated E2-25K (Ubn (K48-linked)-E2-25K) is a polyubiquitinated substrate in which various K48-linked polyUb chains were conjugated on the Ub conjugating enzyme E2-25K (GST tagged). It can be used as a substrate to monitor deubiquitination mediated by deubiquitinating enzymes, or to determine binding of K48-linked polyUb chains by proteins that have Ub interacting domains.

Additional Information

Product Name: Polyubiquitinated E2-25K
Also Known As: Ubn (K48-linked)-E2-25K
Catalog No.: G7000
Size: 50 µg
Molecular Weight: N/A
Species: Human
Source: Bacterial recombinant
Stock: 20 mM Tris, 150 mM NaCl, 2 mM βME, 10% Glycerol
Concentration: See tube label
Quality Assurance: >90% by SDS-PAGE, see datasheet for gel image
Storage: Store at -80°C; avoid multiple freeze-thaw cycles
PDF Data Sheet: PDF DatasheetMSDS
NCBI RefSeq: N/A
Image(s):

Images:
(Click image to enlarge)

Coomassie-stained SDS-PAGE
Lane 1: Molecular weight markers
Lane 2: 5 µg purified polyubiquitinated GST-E2-25K
Shipping Method: Dry ice shipping
References: N/A

Details

Polyubiquitinated E2-25K (Ubn (K48-linked)-E2-25K) is a polyubiquitinated substrate in which various K48-linked polyUb chains were conjugated on the Ub conjugating enzyme E2-25K (GST tagged). It can be used as a substrate to monitor deubiquitination mediated by deubiquitinating enzymes, or to determine binding of K48-linked polyUb chains by proteins that have Ub interacting domains.

Images:
(Click image to enlarge)

Coomassie-stained SDS-PAGE
Lane 1: Molecular weight markers
Lane 2: 5 µg purified polyubiquitinated GST-E2-25K