Ac-Tyr-Val-Ala-Asp-AFC (Ac-YVAD-AFC)

Ac-YVAD-AFC is a fluorogenic substrate of caspase-1/4. Working concentration of this substrate is 25-50 µM. The released AFC (7-Amino-4-trifluoromethylcoumarin) fluorescence can be detected by a fluorimeter or plate reader using excitation/emission wavelengths at 400 nm/505 nm, respectively.

Additional Information

Product Name: Ac-Tyr-Val-Ala-Asp-AFC (Ac-YVAD-AFC)
Also Known As: Ac-YVAD-AFC, Caspase-1/4 substrate
Catalog No.: G5400
Size: 5 mg
Molecular Weight: 719.7 Da
Species: N/A
Source: Synthetic
Stock: Powder
Concentration: N/A
Quality Assurance: >95% by HPLC
Storage: Eligible for room temperature shipping. Store at -20°C upon receiving; avoid multiple freeze-thaw cycles after dissolving in DMSO.
PDF Data Sheet: Download PDF datasheet, MSDS
NCBI RefSeq: N/A
Image(s): N/A
Shipping Method: Room temperature shipping
References: 1. Poreba M., et al., Cold Spring Harb Perspect Bio. 2013, a008680.

Details

Ac-YVAD-AFC is a fluorogenic substrate of caspase-1/4. Working concentration of this substrate is 25-50 µM. The released AFC (7-Amino-4-trifluoromethylcoumarin) fluorescence can be detected by a fluorimeter or plate reader using excitation/emission wavelengths at 400 nm/505 nm, respectively.