GST-RNF4

RING-finger 4 (RNF4), also known as Snurf, is an E3 ubiquitin ligase. RNF4 is composed of four SUMO interaction motifs (SIMs) at the N-terminal and a RING domain at the C-terminal. The SIM domains prefer binding polySUMO chains; RNF4 functions to ubiquitinate polysumolyated proteins including promyelocytic leukaemia (PML), which target them for proteasomal degradation.

Additional Information

Product Name: GST-RNF4
Also Known As: SLX5; SNURF; RES4-26
Catalog No.: K1100
Size: 50 µg
Molecular Weight: 21.3 kDa
Species: Human
Source: Bacterial recombinant
Stock: 20 mM Tris, 150 mM NaCl, 2 mM βME, 10% Glycerol
Concentration: See tube label
Quality Assurance: ~90% by SDS-PAGE, see datasheet for gel image
Storage: Store at -80°C; avoid multiple freeze-thaw cycles
PDF Data Sheet: PDF DatasheetMSDS
NCBI RefSeq: NM_001185009
Image(s): (Click image to enlarge)

Coomassie-stained SDS-PAGE
Lane 1: Molecular weight markers
Lane 2: 5 µg purified GST-RNF4
Shipping Method: Dry ice shipping
References: 1. Chiariotti L, et al. (1998) Genomics 47(2), 258 – 265.
2. Fedele M, et al. (2000) J Biol Chem 275(11), 7894 – 7901.
3. Hunter T, Sun H (2008) Ernst Schering Found Sump Proc (1), 1 – 16.
4. Tatham MH, et al. (2008) Nat Cell BIol 10(5), 538 – 546.
5. Bruderer R, et al. (2011) EMBO Rep 12(2), 142 – 148.

Details

RING-finger 4 (RNF4), also known as Snurf, is an E3 ubiquitin ligase. RNF4 is composed of four SUMO interaction motifs (SIMs) at the N-terminal and a RING domain at the C-terminal. The SIM domains prefer binding polySUMO chains; RNF4 functions to ubiquitinate polysumolyated proteins including promyelocytic leukaemia (PML), which target them for proteasomal degradation.

Images:
(Click image to enlarge)

Coomassie-stained SDS-PAGE
Lane 1: Molecular weight markers
Lane 2: 5 µg purified GST-RNF4