Additional Information
Product Name: | 6xHis-CHIP | ||
---|---|---|---|
Also Known As: | STUB1; UBOX1; HSPABP2; NY-CO-7; SDCCAG7 | ||
Catalog No.: | K1200 | ||
Size: | 50 µg | ||
Molecular Weight: | 34.9 kDa | ||
Species: | Human | ||
Source: | Bacterial recombinant | ||
Stock: | 20 mM Tris, 150 mM NaCl, 2 mM βME, 10% Glycerol | ||
Concentration: | See tube label | ||
Quality Assurance: | ~95% by SDS-PAGE, see datasheet for gel image | ||
Storage: | Store at -80°C; avoid multiple freeze-thaw cycles | ||
PDF Data Sheet: | PDF Datasheet, MSDS | ||
NCBI RefSeq: | NM_005861 | ||
Image(s): | (Click image to enlarge)
|
||
Shipping Method: | Dry ice shipping | ||
References: | 1. Ballinger CA, et al. (1999) Mol Cell Biol 19(6), 4535 – 4545. 2. Murata S, et al. (2003) Int J Biochem Cell Biol 35(5), 572 – 578. |
Details
The carboxyl terminus of Hsc70-interacting protein (CHIP) is an E3 ubiquitin ligase. CHIP contains two domains, one is its TPR domain on the amino terminus and the other is its U-box domain on the carboxyl terminus. The TPR domain recognizes the chaperones, Hsp70 and Hsp90. While the U-box domain carries out its ubiquitin ligase activity. CHIP often ubiquitinates misfolded proteins bound on the chaperones.
Images:
(Click image to enlarge)
Coomassie-stained SDS-PAGE Lane 1: Molecular weight markers Lane 2: 5 µg purified 6xHis-CHIP |