6xHis-Ube2D1 (UbcH5A)

Ube2D1 is an E2 enzyme, which is part of the E1, E2, and E3 cascade responsible for ubiquitination of protein substrates. Two other homologues of Ube2D1 exist called Ube2D2 and Ube2D3. Ube2D2 is 89% identical to the amino acid sequence of Ube2D1 ad Ube2D3 is 88% identical. Ube2D1 has been linked to cadminum toxicity. It might also play a role in LDL cholesterol receptor ubiquitination in an Idol-dependent fashion.

Additional Information

Product Name: 6xHis-Ube2D1 (UbcH5A)
Also Known As: USFT; UBCH5; UBC4/5; UBCH5A; E2(17)KB1
Catalog No.: C1400
Size: 25 µg
Molecular Weight: 16.6 kDa
Species: Human
Source: Bacterial recombinant
Stock: 20 mM Tris, 150 mM NaCl, 2 mM βME, 10% Glycerol
Concentration: See tube label
Quality Assurance: ~95% by SDS-PAGE, see datasheet for gel image
Storage: Store at -80°C; avoid multiple freeze-thaw cycles
PDF Data Sheet: PDF DatasheetMSDS
NCBI RefSeq: NM_003338
Image(s): (Click image to enlarge)

Coomassie-stained SDS-PAGE
Lane 1: Molecular weight markers
Lane 2: 5 µg purified 6xHis-Ube2D1 (UbcH5A)
Shipping Method: Dry ice shipping
References: 1. Scheffner M, et al. (1994) Proc Natl Acad Sci USA 91, 8797–8801.
2. Huibregtse JM, et al. (1995) Proc Natl Acad Sci USA 92(7), 2563 – 2567.
3. Gehrke SG, et al. (2003) Blood 101, 3288 – 3293.
4. Tokumoto M, et al. (2011) J Toxicol Sci 36(2), 191 – 200.

Details

Ube2D1 is an E2 enzyme, which is part of the E1, E2, and E3 cascade responsible for ubiquitination of protein substrates. Two other homologues of Ube2D1 exist called Ube2D2 and Ube2D3. Ube2D2 is 89% identical to the amino acid sequence of Ube2D1 ad Ube2D3 is 88% identical. Ube2D1 has been linked to cadminum toxicity. It might also play a role in LDL cholesterol receptor ubiquitination in an Idol-dependent fashion.

Images:
(Click image to enlarge)

Coomassie-stained SDS-PAGE
Lane 1: Molecular weight markers
Lane 2: 5 µg purified 6xHis-Ube2D1 (UbcH5A)