6xHis-USP16

USP16 is an active deubiquitinating enzyme (DUB) encoded on chromosome 21, trisomy of which causes Down’s syndrome (DS). USP16 is a phosphoprotein, phosphorylation of USP16 controls its cellular localization, but no effect on its DUB activity. USP16 deubiquitinates histone 2A (H2A), through which USP16 might regulate gene expression and cell cycle progression. Increased USP16 protein levels in people with DS might contribute to the development of certain phenotypes of DS.

Additional Information

Product Name: 6xHis-USP16
Also Known As: UBP-M; UBPM
Catalog No.: H6600
Size: 25 µg
Molecular Weight: 93.5 kDa
Species: Human
Source: Insect cell
Stock: 20 mM Tris, 150 mM NaCl, 2 mM βME, 10% Glycerol
Concentration: See tube label
Quality Assurance: >90% by SDS-PAGE, see datasheet for gel image
Storage: Store at -80°C; avoid multiple freeze-thaw cycles
PDF Data Sheet: PDF DatasheetMSDS
NCBI RefSeq: NM_006447.2
Image(s): H6600 image

A. DUB activity assay: 15 nM USP16 + 500 nM Ub-Rhodamine 110. Rhodamine 110 fluorescence was monitored by a plate reader with excitation/emission wavelengths at 480/528 nm, respectively.
Shipping Method: Dry ice shipping
References: 1. Joo H Y.,et al. (2007) Nature 449, 1068.
2. Adorno M., et al. (2013) Nature 501, 380.

Details

USP16 is an active deubiquitinating enzyme (DUB) encoded on chromosome 21, trisomy of which causes Down’s syndrome (DS). USP16 is a phosphoprotein, phosphorylation of USP16 controls its cellular localization, but no effect on its DUB activity. USP16 deubiquitinates histone 2A (H2A), through which USP16 might regulate gene expression and cell cycle progression. Increased USP16 protein levels in people with DS might contribute to the development of certain phenotypes of DS.