5X DUB Assay Buffer II

5X Deubiquitination Buffer II contains 200 mM Tris, pH 7.1 at 37 0C, 500 mM NaCl, 20 mM TCEP and 0.05% Tween-20.

Dilute to 1X when setting up deubiquitination reactions. All tested DUBs (> 25) maintained full activity in the presence of 0.01% Tween-20. We recommend use this buffer for DUB chemical inhibitor screening assay because 0.01% Tween-20 can help solubilize chemicals and prevent non-specific binding of chemicals or DUBs to tube or well wall.

Additional Information

Product Name: 5X DUB Assay Buffer II
Also Known As: Deubiquitination Reaction Buffer II
Catalog No.: H1001
Size: 5 ml
Molecular Weight: N/A
Species: N/A
Source: N/A
Stock: N/A
Concentration: N/A
Quality Assurance: N/A
Storage: Aliquot if necessary; keep frozen for long-term storage.
PDF Data Sheet: PDF Datasheet
NCBI RefSeq: N/A
Image(s): Please see PDF datasheet
Shipping Method: Room temperature shipping
References: No

Details

5X Deubiquitination Buffer II contains 200 mM Tris, pH 7.1 at 37 0C, 500 mM NaCl, 20 mM TCEP and 0.05% Tween-20. Dilute to 1X when setting up deubiquitination reactions. All tested DUBs (> 25) maintained full activity in the presence of 0.01% Tween-20. We recommend use this buffer for DUB chemical inhibitor screening assay because 0.01% Tween-20 can help solubilize chemicals and prevent non-specific binding of chemicals or DUBs to tube or well wall.