Additional Information
| Product Name: | 6xHis-Ubiquitin (K29 only) |
|---|---|
| Also Known As: | 6xHis-[K29-only]Ub |
| Catalog No.: | E1850 |
| Size: | 1 mg |
| Molecular Weight: | 8.5 kDa |
| Species: | Human |
| Source: | Bacterial recombinant |
| Stock: | 20 mM Tris, 150 mM NaCl, 2 mM βME, 10% Glycerol |
| Concentration: | See tube label |
| Quality Assurance: | ~95% by SDS-PAGE, see datasheet for gel image |
| Storage: | Store at -80°C; avoid multiple freeze-thaw cycles |
| PDF Data Sheet: | PDF Datasheet， MSDS |
| NCBI RefSeq: | N/A |
| Image(s): | No |
| Shipping Method: | Dry ice shipping |
| References: | 1. Hershko A, et al. (1980) Proc Natl Acad Sci USA 77(4), 1783 – 1786. 2. Pickart CM (1997) FASEB 11(13), 1055 – 1066. 3. Hershko A, et al. (1998) Ann Rev Biochem 67, 425 – 479. 4. Jiang X, et al. (2012) Nature Reviews Immunology 12, 35 – 48. 5. Bremm A, et al. (2012) Methods Mol Biol 832, 291 – 228. |
Details
Ubiquitin (Ub) is a 76 amino acid protein widely expressed in the cytoplasmic and nucleus of cells. Ub is posttranslationally conjugated to proteins by the E1, E2, E3 protein ubiquitination cascade. Ub can be conjugated on proteins as monoUb or polyUb chains. Protein ubiquitination plays both proteolytic and nonproteolytic functions. Usually, polyubiquitinated proteins are targeted to the 26S proteasome for proteolysis. Typical concentration to support in vitro ubiquitination is 50-100 µM. In Ub(K29 only), all lysine residues but lysine 29 were substituted with arginines, thereby it only supports the formation of lysine 29-linked polyubiquitin chains.
Images
(Click image to enlarge)
Coomassie-stained SDS-PAGE Lane 1: Molecular weight markers Lane 2: 5 µg purified 6xHis-Ubiquitin (K0) Lane 3: 5 µg purified 6xHis-Ubiquitin (K6 only) Lane 4: 5 µg purified 6xHis-Ubiquitin (K11 only) Lane 5: 5 µg purified 6xHis-Ubiquitin (K27 only) Lane 6: 5 µg purified 6xHis-Ubiquitin (K29 only) Lane 7: 5 µg purified 6xHis-Ubiquitin (K33 only) Lane 8: 5 µg purified 6xHis-Ubiquitin (K48 only) Lane 9: 5 µg purified 6xHis-Ubiquitin (K63 only)
Coomassie-stained SDS-PAGE Lane 1: Molecular weight markers Lane 2: 5 µg purified 6xHis-Ubiquitin (K0) Lane 3: 5 µg purified 6xHis-Ubiquitin (K6 only) Lane 4: 5 µg purified 6xHis-Ubiquitin (K11 only) Lane 5: 5 µg purified 6xHis-Ubiquitin (K27 only) Lane 6: 5 µg purified 6xHis-Ubiquitin (K29 only) Lane 7: 5 µg purified 6xHis-Ubiquitin (K33 only) Lane 8: 5 µg purified 6xHis-Ubiquitin (K48 only) Lane 9: 5 µg purified 6xHis-Ubiquitin (K63 only)
