Proteasome Activity Fluorometric Assay Kit II - Proteasomes / Activators / Subunits

Additional Information

Product Name:

Proteasome Activity Fluorometric Assay Kit II

Also Known As:

N/A

Catalog No.:

J4120

Size:

1 Kit

Molecular Weight:

N/A

Species:

N/A

Source:

N/A

Stock:

N/A

Concentration:

See tube label

Quality Assurance:

Inhibitor and substrate > 98% by HPLC and NMR; activity assay using cell lysates.

Storage:

Store at -80°C; avoid multiple freeze-thaw cycles

PDF Data Sheet:

PDF datasheet, protocol; MSDS

NCBI RefSeq:

N/A

Image(s):

J4120 Image:
(Click image to enlarge)

AMC fluorescence recorded in reactions containing 50 µg HEK293T cell lysates, 50 µM Suc-LLVY-AMC, Z-LLE-AMC or Boc-LRR-AMC, in the presence of absence of 100 µM MG132.

Shipping Method:

Dry ice shipping

References:

N/A

Details

Each supplied fluorogenic substrate is sufficient for 250 x 100 µl reactions.

Description

Proteasome Activity Fluorometric Assay Kit II was designed for assaying the constitutive proteasomes' chymotrypsin-like, trypsin-like and caspase-like activities in vitro using purified proteasome, cell lysates or tissue extracts. The proteasome cleaves the supplied fluorogenic substrates and the released AMC fluorescence can be monitored using a plate reader or fluorometer at the excitation/emission wavelength of 360nm/460nm, respectively.

Suc-LLVY-AMC, Boc-LRR-AMC and Z-LLE-AMC are used to monitor the chymotrypsin-like, trypsin-like and caspase-like activities of the proteasome, respectively.

Components

• AMC (0.1 mM in DMSO)....................................................30 µl
• 1000X SUC-LLVY-AMC (50 mM in DMSO)........................25 µl
• 1000X BOC-LRR-AMC (50 mM in DMSO)........................25 µl
• 1000X Z-LLE-AMC (50 mM in DMSO)...............................25 µl
• HEK293T cell lysates (5mg/ml)..........................................100 µl
• MG-132..............................................................................1 mg
• DMSO................................................................................200 µl
• ATP (0.5M).........................................................................100 µl
• MgCl₂ (1M)....................................................250 µl
• 20X Proteasome Assay Buffer........................................3x 1.35 ml