Details
Each supplied substrate is sufficient for 250 X 100 µl reactions.
Description
Immunoproteasome Activity Fluorometric Assay Kit II was designed for assaying the immunoproteasomes' chymotrypsin-like, trypsin-like and branched amino acid preferring activities in vitro using purified proteasome, cell lysates or tissue extracts. The proteasome cleaves these fluorogenic substrates, and the released AMC fluorescence can be monitored using a plate reader or fluorometer at the excitation/emission wavelength of 360nm/460nm, respectively.
Components
• AMC (0.1mM in DMSO)................................................30 µl
• 1000X Ac-ANW-AMC (50 mM in DMSO)......................25 µl
• 1000X Ac-PAL-AMC (50 mM in DMSO)......................25 µl
• 1000X Ac-KQL-AMC (50 mM in DMSO)......................25 µl
• Mouse spleen extracts (5 mg/ml)..................................100 µl
• MG-132..........................................................................1 mg
• DMSO............................................................................200 µl
• ATP (0.5 M)....................................................................100 µl
• MgCl2 (1 M)...............................................250 µl
• 20X Proteasome Assay Buffer........................................3 X 1.35 ml