Additional Information
Product Name: | CHIP Rabbit pAb |
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Catalog No.: | Y6232-100 |
Also Known As: | STUB1; CHIP; HSPABP2; NY-CO-7; SCAR16; SDCCAG7; UBOX1 |
Size: | 100 µl |
Concentration: | See labels on tube |
Host Species: | Rabbit |
Isotype: | IgG |
Reactivity: | Human, Mouse, Rat |
Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 1-303 of human CHIP (NP_005852.2). |
Swiss Prot. #: | Q9UNE7 |
Calculated MW: | 34 kDa |
Detected MW: | 34 kDa |
Applications: | WB (1:500 - 1:2,000) IF (1:50 - 1:200) IP (not tested) IHC (not tested) Note: Antibody dilution should be optimized by users. |
Purification: | Protein A or G affinity purification |
Buffer: | PBS with 0.02% sodium azide, 50% glycerol, pH7.3 |
Storage: | Store at -20℃. Centrifuge to maximize product recovery. |
PDF Data Sheet: | PDF Datasheet, MSDS |
Image(s): | (Click image to enlarge) |
Shipping Method: | Wet ice shipping |
References: | 1. McDonough H and Patterson C, (2003) Cell Stress Chaperones 8, 303 - 308. 2. Murata S, et al. (2001) EMBO Rep 2, 1133 - 1138. |
Details
CHIP is a co-chaperone protein and functional E3 ubiquitin ligase that links the polypeptide binding activity of Hsp70 to the ubiquitin proteasome system. CHIP contains three 34-amino acid TPR (tetratricopeptide repeat) domains at its amino terminus and a carboxy-terminal U-box domain. CHIP interacts with the molecular chaperones Hsc70-Hsp70 and Hsp90 through its TPR domain, while E3 ubiquitin ligase activity is confined to the U-box domain.