Additional Information
| Product Name: | Ub aldehyde |
|---|---|
| Also Known As: | Ubiquitin aldehyde, Ubal |
| Catalog No.: | F3100 |
| Size: | 50 µg |
| Molecular Weight: | 9 kDa |
| Species: | Human |
| Source: | Bacterial recombinant |
| Stock: | 20 mM sodium acetate, pH 5.0 |
| Concentration: | See tube label |
| Quality Assurance: | ~95% by SDS-PAGE, see datasheet for gel image |
| Storage: | Store at -80°C; avoid multiple freeze-thaw cycles |
| PDF Data Sheet: | PDF Datasheet， MSDS |
| NCBI RefSeq: | N/A |
| Image(s): | Click image to enlarge
 Open circle: 250 nM Ub-Rhodamine 110 Solid triangle: 250 nM Ub-Rhodamine 110 + 5 nM GST-USP4 Solid circle: 250 nM Ub-Rhodamine 110 + 5 nM GST-USP4 + 2 μM Ubal (overlapped with open circles) Reaction buffer: 40 mM Tris, pH 7.1 at 37°C, 150 mM NaCl, 5 mM DTT and 10% glycerol |
| Shipping Method: | Dry ice shipping |
| References: | 1. Hershko A,et al. (1987) Proc Natl Acad Sci 84, 1829 – 1833. 2. Hadari T, et al. (1992) J Biol Chem 267(2), 719 – 727. 3. Melandri F, et all. (1996 Biochemistry 35(39), 12893 – 12900. 4. Hu, M. et al. (2005) EMBO J. 24. 3747. |
Details
Ub aldehyde is a potent and highly specific inhibitor of cysteine deubiquitinating enzymes including the Ub C-terminal hydrolases (UCHs), the Ub-specific proteases (USPs), the ovarian tumor domains (OTUs) proteases and the Machado-Josephine domain (MJD) proteases. Typical working concentration is 2-5 µM in vitro.
Images
(Click image to enlarge)
Open circle: 250 nM Ub-Rhodamine 110
Solid triangle: 250 nM Ub-Rhodamine 110 + 5 nM GST-USP4
Solid circle: 250 nM Ub-Rhodamine 110 + 5 nM GST-USP4 + 2 μM Ubal (overlapped with open circles)
Reaction buffer: 40 mM Tris, pH 7.1 at 37°C, 150 mM NaCl, 5 mM DTT and 10% glycerol
Open circle: 250 nM Ub-Rhodamine 110
Solid triangle: 250 nM Ub-Rhodamine 110 + 5 nM GST-USP4
Solid circle: 250 nM Ub-Rhodamine 110 + 5 nM GST-USP4 + 2 μM Ubal (overlapped with open circles)
Reaction buffer: 40 mM Tris, pH 7.1 at 37°C, 150 mM NaCl, 5 mM DTT and 10% glycerol
