Ub-AMC

Ubiquitin 7-amido-4-methylcoumarin (Ub-AMC) is a sensitive fluorescence-based substrate of deubiquitinating enzymes. It is useful for monitoring deubiquitinating enzymes’ activity or screening inhibitors of deubiquitinating enzymes. The concentration of Ub-AMC used in deubiquitinating reactions is usually 0.1-1 µM. The released AMC fluorescence can be monitored using a fluorometer or plate reader with excitation and emission wavelengths at 360 nm and 460 nm, respectively.

Additional Information

Product Name: Ub-AMC
Also Known As: Ubiquitin-AMC
Catalog No.: M3010
Size: 50 µg
Molecular Weight: 9 kDa
Species: Human
Source: Bacterial recombinant
Stock: DMSO
Concentration: See tube label
Quality Assurance: >95% by SDS-PAGE
Storage: Store at -80°C; avoid multiple freeze-thaw cycles
PDF Data Sheet: PDF Datasheet
NCBI RefSeq: N/A
Image(s): No
Shipping Method: Dry ice shipping
References: Wilkinson KD, et al. (2005) Methods Enzymol. 399, 37-51

Details

Ubiquitin 7-amido-4-methylcoumarin (Ub-AMC) is a sensitive fluorescence-based substrate of deubiquitinating enzymes. It is useful for monitoring deubiquitinating enzymes’ activity or screening inhibitors of deubiquitinating enzymes. The concentration of Ub-AMC used in deubiquitinating reactions is usually 0.1-1 µM. The released AMC fluorescence can be monitored using a fluorometer or plate reader with excitation and emission wavelengths at 360 nm and 460 nm, respectively.

Images
(Click image to enlarge)


Coomassie-stained SDS-PAGE
5 µg purified Ub-AMC


The reaction contained 2 nM bovine 26S proteasome and 1 mM Ub-AMC. AMC fluorescence was monitored by a plate reader using excitation and emission filter set at 360/40 and 460/30 nm, respectively.