TAMRA-Lys(Ub)-Gly

This products is a fluorescence polarization (FP) assay reagent, which is based on a 5-carboxytetramethylrhodamine (TAMRA) modified Lys-Gly sequence that is linked to ubiquitine via a native isopeptide bond with the lysine side-chain. Typical substrate concentrations range from 10−100 nM. DUB concentrations can range from 0.01-10 nM but depend on specific assay conditions and method of detection. FP assays can be performed with any fluorescence polarization reader with a 530 nm excitation filter and two 580 nm emission filters.

Additional Information

Product Name: TAMRA-Lys(Ub)-Gly
Also Known As: N/A
Catalog No.: M3030
Size: 50 µg
Molecular Weight: 9163 Da by MS
Species: Human
Source: Synthetic
Stock: Product in lyophilized powder form.
Concentration: N/A
Quality Assurance: ≥95% by RP-HPLC and SDS-PAGE
Storage: Powder at −20°C; solution at −80°C. Avoid multiple freeze/thaw cycles.
PDF Data Sheet: PDF DatasheetMSDS
NCBI RefSeq: N/A
Image(s): (Click image to enlarge)

Shipping Method: Room temperature shipping
References: 1. Tirat, A. et al., (2005) Anal. Biochem. 343, 244.
2. Huang et al., (2009) Methods in Molecular Biology 565, 127.
3. Levine et al., (1997) Anal. Biochem. 247, 83.
4. Geurink and El Oualid et al., (2012) ChemBiochem 13, 293.

Details

This products is a fluorescence polarization (FP) assay reagent, which is based on a 5-carboxytetramethylrhodamine (TAMRA) modified Lys-Gly sequence that is linked to ubiquitine via a native isopeptide bond with the lysine side-chain. Typical substrate concentrations range from 10−100 nM. DUB concentrations can range from 0.01-10 nM but depend on specific assay conditions and method of detection. FP assays can be performed with any fluorescence polarization reader with a 530 nm excitation filter and two 580 nm emission filters.

Sample Preparation:
Dissolve the powder in DMSO to a final concentration of 1mM (50 μg/5.5 μl) and add this DMSO stock slowly to the required buffer (please note the order of addition) to a final concentration of 0.1 - 1 μM. The concentration of 5-TAMRA-Lys(Ub)-Gly-OH can be verified by comparing the fluorescence intensity with that of a known concentration of TAMRA.

Images:
(Click image to enlarge)