
Additional Information
Product Name: | TAMRA-Lys(Ub)-Gly | |
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Also Known As: | N/A | |
Catalog No.: | M3030 | |
Size: | 50 µg | |
Molecular Weight: | 9163 Da by MS | |
Species: | Human | |
Source: | Synthetic | |
Stock: | Product in lyophilized powder form. | |
Concentration: | N/A | |
Quality Assurance: | ≥95% by RP-HPLC and SDS-PAGE | |
Storage: | Powder at −20°C; solution at −80°C. Avoid multiple freeze/thaw cycles. | |
PDF Data Sheet: | PDF Datasheet, MSDS | |
NCBI RefSeq: | N/A | |
Image(s): | (Click image to enlarge)
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Shipping Method: | FedEx | |
References: | 1. Tirat, A. et al., (2005) Anal. Biochem. 343, 244. 2. Huang et al., (2009) Methods in Molecular Biology 565, 127. 3. Levine et al., (1997) Anal. Biochem. 247, 83. 4. Geurink and El Oualid et al., (2012) ChemBiochem 13, 293. |
Details
This products is a fluorescence polarization (FP) assay reagent, which is based on a 5-carboxytetramethylrhodamine (TAMRA) modified Lys-Gly sequence that is linked to ubiquitine via a native isopeptide bond with the lysine side-chain. Typical substrate concentrations range from 10−100 nM. DUB concentrations can range from 0.01-10 nM but depend on specific assay conditions and method of detection. FP assays can be performed with any fluorescence polarization reader with a 530 nm excitation filter and two 580 nm emission filters.
Sample Preparation:
Dissolve the powder in DMSO to a final concentration of 1mM (50 μg/5.5 μl) and add this DMSO stock slowly to the required buffer (please note the order of addition) to a final concentration of 0.1 - 1 μM. The concentration of 5-TAMRA-Lys(Ub)-Gly-OH can be verified by comparing the fluorescence intensity with that of a known concentration of TAMRA. Images: (Click image to enlarge)
Sample Preparation:
Dissolve the powder in DMSO to a final concentration of 1mM (50 μg/5.5 μl) and add this DMSO stock slowly to the required buffer (please note the order of addition) to a final concentration of 0.1 - 1 μM. The concentration of 5-TAMRA-Lys(Ub)-Gly-OH can be verified by comparing the fluorescence intensity with that of a known concentration of TAMRA. Images: (Click image to enlarge)
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