Cy5-Ub-VME

Cy5-Ub-VME is a potent, irreversible and specific inhibitor of deubiquitinating enzymes (DUBs), which is labeled on the N-terminus with a Cy5 dye (Cy5, Ex 625-650 nm, Em 670 nm). This ubiquitin-based activity probe can be used for activity profiling experiments and labeling of DUBs with a fluorescent dye (Cy5). Cy5 labelling allows for detection of DUB labeling by in-gel fluorescence.

Additional Information

Product Name: Cy5-Ub-VME
Also Known As: N/A
Catalog No.: M1040
Size: 25 µg
Molecular Weight: 9.1 kDa
Species: Human
Source: Synthetic
Stock: Product in lyophilized powder form.
Concentration: N/A
Quality Assurance: ≥95% by RP-HPLC
Storage: Powder at −20°C; solution at −80°C. Avoid multiple freeze/thaw cycles.
PDF Data Sheet: PDF DatasheetMSDS
NCBI RefSeq: N/A
Image(s): No
Shipping Method: Room temperature shipping
References: 1. Altun et al., (2011) Chem Biol 18, 1401.
2. Misaghi et al., (2005) J. Biol. Chem. 280, 1512.

Details

Cy5-Ub-VME is a potent, irreversible and specific inhibitor of deubiquitinating enzymes (DUBs), which is labeled on the N-terminus with a Cy5 dye (Cy5, Ex 625-650 nm, Em 670 nm). This ubiquitin-based activity probe can be used for activity profiling experiments and labeling of DUBs with a fluorescent dye (Cy5). Cy5 labelling allows for detection of DUB labeling by in-gel fluorescence.

Sample Preparation:
Dissolve the powder in DMSO (e.g. add 1.25 μl DMSO to 25 μg powder to get 20 mg/ml or 2.25 mM DMSO stock) and add this DMSO stock to the required buffer (please note the order of addition). Typical reaction concentrations vary from 0.2 - 2 μM.