Biotin-Ub-PA

Biotin-Ub-PA is a newly developed potent and specific inhibitor of deubiquitinating enzymes (DUBs), which has a N-terminal biotin tag. An aminohexanoic acid (Ahx) linker is used to create extra space between the biotin and Ub protein for efficient access of the biotin binding entity. This activity probe can be used for activity profiling experiments and determining DUB inhibitor specificity. It has two unique capabilities: it forms a covalent linkage with the active site Cys residue of a DUB that can be cleaved by acid treatment (5% aq. TFA), allowing for proteomic analyses. Biotin-Ub-PA targets all three major DUB families: UCH, USP and OTU

Additional Information

Product Name: Biotin-Ub-PA
Also Known As: N/A
Catalog No.: M1020
Size: 25 µg
Molecular Weight: 8885 Da by MS
Species: Human
Source: Synthetic
Stock: Product in lyophilized powder form.
Concentration: N/A
Quality Assurance: ≥95% by RP-HPLC and SDS-PAGE
Storage: Powder at −20°C; solution at −80°C. Avoid multiple freeze/thaw cycles.
PDF Data Sheet: PDF DatasheetMSDS
NCBI RefSeq: N/A
Image(s): (Click image to enlarge)

SDS-PAGE analysis.
Coommassie blue staining, 12% SDS-PAGE gel.
Shipping Method: Room temperature shipping
References: 1. Ekkebus et al., (2013) J. Am. Chem. Soc. 135, 2867.
2. Sommer et al., (2013) Bioorg. Med. Chem. 21, 2511.
3. Galardy et al., (2005) Methods in Enzymology 399, 120.
4. de Jong et al., (2012) ChemBioChem 13, 2251.

Details

Biotin-Ub-PA is a newly developed potent and specific inhibitor of deubiquitinating enzymes (DUBs), which has a N-terminal biotin tag. An aminohexanoic acid (Ahx) linker is used to create extra space between the biotin and Ub protein for efficient access of the biotin binding entity. This activity probe can be used for activity profiling experiments and determining DUB inhibitor specificity. It has two unique capabilities: it forms a covalent linkage with the active site Cys residue of a DUB that can be cleaved by acid treatment (5% aq. TFA), allowing for proteomic analyses. Biotin-Ub-PA targets all three major DUB families: UCH, USP and OTU

Sample Preparation:
Dissolve the powder in DMSO (e.g. add 1.25 μl DMSO to 25 μg powder to get 20 mg/ml or 2.25 mM DMSO stock) and add this DMSO stock slowly to the required buffer (please note the order of addition).Typic reaction concentrations vary from 0.2 -2 mM.

Images:
(Click image to enlarge)

SDS-PAGE analysis.
Coommassie blue staining, 12% SDS-PAGE gel.