
Additional Information
Product Name: | Biotin-Ub-PA | ||
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Also Known As: | N/A | ||
Catalog No.: | M1020 | ||
Size: | 25 µg | ||
Molecular Weight: | 8885 Da by MS | ||
Species: | Human | ||
Source: | Synthetic | ||
Stock: | Product in lyophilized powder form. | ||
Concentration: | N/A | ||
Quality Assurance: | ≥95% by RP-HPLC and SDS-PAGE | ||
Storage: | Powder at −20°C; solution at −80°C. Avoid multiple freeze/thaw cycles. | ||
PDF Data Sheet: | PDF Datasheet, MSDS | ||
NCBI RefSeq: | N/A | ||
Image(s): | (Click image to enlarge)
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Shipping Method: | FedEx | ||
References: | 1. Ekkebus et al., (2013) J. Am. Chem. Soc. 135, 2867. 2. Sommer et al., (2013) Bioorg. Med. Chem. 21, 2511. 3. Galardy et al., (2005) Methods in Enzymology 399, 120. 4. de Jong et al., (2012) ChemBioChem 13, 2251. |
Details
Biotin-Ub-PA is a newly developed potent and specific inhibitor of deubiquitinating enzymes (DUBs), which has a N-terminal biotin tag. An aminohexanoic acid (Ahx) linker is used to create extra space between the biotin and Ub protein for efficient access of the biotin binding entity. This activity probe can be used for activity profiling experiments and determining DUB inhibitor specificity. It has two unique capabilities: it forms a covalent linkage with the active site Cys residue of a DUB that can be cleaved by acid treatment (5% aq. TFA), allowing for proteomic analyses. Biotin-Ub-PA targets all three major DUB families: UCH, USP and OTU
Sample Preparation:
Dissolve the powder in DMSO (e.g. add 1.25 μl DMSO to 25 μg powder to get 20 mg/ml or 2.25 mM DMSO stock) and add this DMSO stock slowly to the required buffer (please note the order of addition).Typic reaction concentrations vary from 0.2 -2 mM. Images: (Click image to enlarge)
Sample Preparation:
Dissolve the powder in DMSO (e.g. add 1.25 μl DMSO to 25 μg powder to get 20 mg/ml or 2.25 mM DMSO stock) and add this DMSO stock slowly to the required buffer (please note the order of addition).Typic reaction concentrations vary from 0.2 -2 mM. Images: (Click image to enlarge)
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SDS-PAGE analysis. Coommassie blue staining, 12% SDS-PAGE gel. |