Biotin-SUMO2-VME

A potent, irreversible and specific inhibitor of deSUMOylation proteases (SENPs) that cleave SUMO2 conjugates. It is N-terminally tagged with a biotin, which allows for sensitive identification of SENPs using HRP-conjugated streptavidin in immunoblotting assays, or purification of SENPs using streptavidin resin. The biotin tag is separated from SUMO2 by an aminohexanoic acid (Ahx) linker that could increase the reactivity of the biotin moiety. In addition, Cys48 in SUMO2 was mutated to Ser.

Additional Information

Product Name: Biotin-SUMO2-VME
Also Known As: N/A
Catalog No.: M2010
Size: 25 µg
Molecular Weight: 11.02 kDa
Species: Human
Source: Synthetic
Stock: Product in lyophilized powder form.
Concentration: N/A
Quality Assurance: ≥95% by RP-HPLC
Storage: Powder at −20°C; solution at −80°C. Avoid multiple freeze/thaw cycles.
PDF Data Sheet: PDF DatasheetMSDS
NCBI RefSeq: N/A
Image(s): No
Shipping Method: Room temperature shipping
References: 1. Albrow et al., (2011) Chem Biol 18, 722.
2. Mendes et al., (2016) Biochim Biophys Acta - Mol Cell Res. 1863, 139.

Details

A potent, irreversible and specific inhibitor of deSUMOylation proteases (SENPs) that cleave SUMO2 conjugates. It is N-terminally tagged with a biotin, which allows for sensitive identification of SENPs using HRP-conjugated streptavidin in immunoblotting assays, or purification of SENPs using streptavidin resin. The biotin tag is separated from SUMO2 by an aminohexanoic acid (Ahx) linker that could increase the reactivity of the biotin moiety. In addition, Cys48 in SUMO2 was mutated to Ser.

Sample Preparation:
Brief centrifugation at 10,000 xg for 2 minutes to pellet the powder. Dissolve the powder in small amount of DMSO (e.g. 25 μg in 0.625 μL = 40 μg/μL). Add this DMSO stock slowly to 50 μL buffer such as 20 mM Tris, pH 7.2, 50 mM NaCl to make a stock solution of 0.5 μg/μL (45 μM) (please note the order of addition). Tap the tube to mix. Working concentration 0.5-2 μM.