Additional Information
| Product Name: | Ubiquitin Linkage Screening Kit III |
|---|---|
| Also Known As: | N/A |
| Catalog No.: | J3220 |
| Size: | 1 Kit |
| Molecular Weight: | N/A |
| Species: | Human |
| Source: | Bacterial recombinant |
| Stock: | 20 mM Tris, 150 mM NaCl, 2 mM βME, 10% Glycerol |
| Concentration: | See tube label |
| Quality Assurance: | 95% based on SDS-PAGE |
| Storage: | Store at -80°C; avoid multiple freeze-thaw cycles |
| PDF Data Sheet: | PDF datasheet, MSDS |
| NCBI RefSeq: | N/A |
| Image(s): | No |
| Shipping Method: | Dry ice shipping |
| References: | N/A |
Details
* The supplied UBE1 is sufficient for setting up 50 x 20 µl reactions. Each Ub mutant is sufficient for setting up 10 X 20 µl reactions.
Description
Ubiquitin Linkage Screening Kit III is designed for two purposes: 1) to identify the ubiquitin lysine residue that is used to link the polyubiquitin chain conjugated on a protein substrate; 2) to determine whether a protein substrate is polyubiquitinated or conjugated with multiple monoubiquitin.
A typical 20 µl reaction contains 100 nM UBE1, 2 µM E2 (not supplied), 50 µM Ub or Ub mutants, 4 mM ATP, 2 µM E3 (not supplied) and 2 µM substrate (no supplied) in the ubiquitination buffer.
Components
• 20X UBE1 (2 µM)..................................50 µl
• 10X 6xHis-Ub (500 µM)..........................20 µl
• 10X 6xHis-Ub(K0) (500 µM)....................20 µl
• 10X 6xHis-Ub(K6 only) (500 µM).............20 µl
• 10X 6xHis-Ub(K11 only) (500 µM)............20 µl
• 10X 6xHis-Ub(K27 only) (500 µM)............20 µl
• 10X 6xHis-Ub(K29 only) (500 µM)............20 µl
• 10X 6xHis-Ub(K33 only) (500 µM)............20 µl
• 10X 6xHis-Ub(K48 only) (500 µM)............20 µl
• 10X 6xHis-Ub(K63 only) (500 µM)............20 µl
• 10X ATP (40 mM)....................................125 µl
• 10X Ubiquitination Buffer...........................1 ml
