Additional Information
Product Name: | Linear polyUb Chain-binding Protein Identification / Validation Kit |
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Also Known As: | N/A |
Catalog No.: | J2520 |
Size: | 1 Kit |
Molecular Weight: | N/A |
Species: | Human |
Source: | Bacterial recombinant |
Stock: | 20 mM Tris, 150 mM NaCl, 2 mM βME, 10% Glycerol |
Concentration: | See tube label |
Quality Assurance: | 95% based on SDS-PAGE |
Storage: | See the storage condition on the label of each component; avoid multiple freeze-thaw cycles |
PDF Data Sheet: | PDF datasheet |
NCBI RefSeq: | N/A |
Image(s): | No |
Shipping Method: | Dry ice shipping |
References: | N/A |
Details
* This kit is designed for two purposes: 1) identification of proteins that specially bind linear polyUb chains in whole cell or tissue lysates; 2) validation of direct interactions between linear polyUb chains and their binding proteins.
Description
The non-cleavable 6xHis-tagged linear Ub6 is resistant of deubiquitination when incubating with whole cell or tissue lysates, thus allowing maximally capture proteins that specifically bind linear polyUb chains. After binding, 6XHis-tagged non-cleavable linear Ub6 and the binding proteins can be enriched with Nickel XPure Agarose Resin (included). The bound proteins and the non-cleavable polyUb chains can be eluted from Ni resin using a buffer containing 250 mM imidazole (included) or by incubating with TEV protease (not included). Either of the elution method can significantly reduce the amounts of non-specific binding proteins when compared with elution using SDS sample buffer.
Components
• 6xHis-Non-cleavable Linear Ub 6 [Store at -80°C]..........100 µg
• 6xHis-Ubiquitin [Store at -80°C].............................................................................100 µg
• Nickel XPure Agarose Resin (suspended in 50% slurry) [Store at 2-8°C]....................500 µl
• 4 M Imidazole [Store at -80°C]......................................................................................500 µl
• Iodoacetamide ( IAA ) [Store at -80°C]..........................................................................25 mg