Additional Information
Product Name: | K48-Ub4 |
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Also Known As: | K48-Ub4 |
Catalog No.: | D1301 |
Size: | 50 µg |
Molecular Weight: | 33.1 kDa |
Species: | Human |
Source: | Bacterial recombinant |
Stock: | 20 mM Tris, 150 mM NaCl, 2 mM βME, 10% Glycerol |
Concentration: | See tube label |
Quality Assurance: | ~90% by SDS-PAGE, see datasheet for gel image |
Storage: | Store at -80°C; avoid multiple freeze-thaw cycles |
PDF Data Sheet: | PDF Datasheet, MSDS |
NCBI RefSeq: | N/A |
Image(s): | Coomassie stained SDS-PAGE Lane 1: Marker Lane 2: K48 Ub2 Lane 3: K48 Ub3 Lane 4: K48 Ub4 Lane 5: K48 Ub5 Lane 6: K48 Ub2-8 |
Shipping Method: | Dry ice shipping |
References: | 1. Hershko A, et al. (1980)Proc Natl Acad Sci USA 77(4), 1783 – 1786. 2. Pickart CM, (1997) FASEB 11(13), 1055 – 1066. 3. Hershko A, et al. (1998) Ann Rev Biochem 67, 425 – 479. 4. Jiang X, et al. (2012) Nature Reviews Immunology 12, 35 – 48. |
Details
Ub chains are formed by conjugating the C-terminal glycine residue of Ub onto any of seven internal lysine residues or the amino group of the previous Ub. Ub chains are classified by the lysine residue used to link Ubs; different Ub chain topologies can result in different signals. For instance, Ub chains linked through lysine 6, 11, 27, 29, 33 and 48 are capable of targeting proteins for proteasomal degradation; in contrast, Ub chains linked through lysine 63 or the N-terminal amino group (linear Ub chains) often play important nonproteolytic functions including regulation of kinase activation and protein translation. All Ub chain products are produced by using of human wild type Ub reacting with specific E2s.
Images
(Click image to enlarge)
Coomassie-stained SDS-PAGE Lane 1: Molecular weight markers Lane 2: 5 µg purified K48-Ub2 Lane 3: 5 µg purified K48-Ub3 Lane 4: 5 µg purified K48-Ub4 Lane 5: 5 µg purified K48-Ub5
Coomassie-stained SDS-PAGE Lane 1: Molecular weight markers Lane 2: 5 µg purified K48-Ub2 Lane 3: 5 µg purified K48-Ub3 Lane 4: 5 µg purified K48-Ub4 Lane 5: 5 µg purified K48-Ub5