K29-linked diUb

This product is a native K29-linked diUb which can be used as a substrate of deubiquitinating enzyems. It can also be used to investigate linkage specificity of proteins that contain ubiquitin-associated domains or ubiquitin-interacting motifs. This product is formed by chemical ligation.

Additional Information

Product Name: K29-linked diUb
Also Known As: N/A
Catalog No.: D5310
Size: 25 µg
Molecular Weight: 17107 Da by MS
Species: Human
Source: Synthetic
Stock: Product in lyophilized powder form
Concentration: N/A
Quality Assurance: ≥95% by RP-HPLC and SDS-PAGE
Storage: Powder at −20°C; solution at −80°C. Please avoid multiple freeze/thaw cycles.
PDF Data Sheet: PDF DatasheetMSDS
NCBI RefSeq: N/A
Image(s): (Click image to enlarge)

Coomassie-stained SDS-PAGE of 1 mg each diUb
A: Linear diUb
B: K6-linked diUb
C: K11-linked diUb
D: K27-linked diUb
E: K29-linked diUb
F: K33-linked diUb
G: K48-linked diUb
H: K63-linked diUb
I: Ubiquitin
Shipping Method: Room temperature shipping
References: 1. A. Faesen et al., (2011) Chemistry & Biology, 18, 1550.
2. I. Dikic et al., (2010) Nature Reviews Molecular Cell Biology 10, 659.
3. J. D. F. Licchesi et al., (2012) Nature Structural & Molecular Biology 19, 62.
4. F. El Oualid et al., (2010) Angewandte Chemie Int. Ed. 49, 10149.

Details

This product is a native K29-linked diUb which can be used as a substrate of deubiquitinating enzyems. It can also be used to investigate linkage specificity of proteins that contain ubiquitin-associated domains or ubiquitin-interacting motifs. This product is formed by chemical ligation.

Sample Preparation:
Dissolve the powder in DMSO (e.g. 50 μg in 2.5 μL = 1169 μM) and add this DMSO stock slowly to the required buffer (please note order of addition). General used concentrations vary from 0.1 – 1 μM.

Images:
(Click image to enlarge)

Coomassie-stained SDS-PAGE of 1 mg each diUb
A: Linear diUb
B: K6-linked diUb
C: K11-linked diUb
D: K27-linked diUb
E: K29-linked diUb
F: K33-linked diUb
G: K48-linked diUb
H: K63-linked diUb
I: Ubiquitin