K29/K33 Polyubiquitin Chain Capture Kit - Ubiquitin / Sumo Chains

Additional Information

Product Name:

K29/K33 Polyubiquitin Chain Capture Kit

Also Known As:

N/A

Catalog No.:

J4470

Size:

1 Kit

Molecular Weight:

N/A

Species:

N/A

Source:

N/A

Stock:

Enzyme stocked in 20 mM Tris, 150 mM NaCl, 2 mM βME, 10% Glycerol

Concentration:

See tube label

Quality Assurance:

Enzyme >95% by SDS-PAGE

Storage:

Store at -80°C; avoid multiple freeze-thaw cycles

PDF Data Sheet:

PDF datasheet, MSDS

NCBI RefSeq:

N/A

Image(s):

Images:
(Click image to enlarge)

A procedure for capturing K29/K33 polyubiquitinated proteins in cell or tissue lysates

GST-TRABID(NZF1) pulldown assay
1. 20 ug cell lysates
2. GST pulldown
3. GST-TRABID(NZF1) pulldown

Shipping Method:

Dry ice shipping

References:

N/A

Details

Description

This kit is designed for enrichment of cellular proteins conjugated with lysine 29/33-linked polyubiquitin chains using GST-TRABID (ZNF1). Polyubiquitinated proteins bound on GST-TRABID (ZNF1) can be precipi-tated using glutathione resin and eluted by a buffer containing 10 mM glutathione. Enriched polyubiqui-tinated proteins can be assayed by immunoblotting or mass spectrometry.

Components

• GST-TRABID (ZNF1) (2 mg/ml each)......................................1 ml
• GST (4 mg/ml)..........................................................................0.5 ml
• Iodoacetamine (IAA).................................................................25 mg
• Glutathione (reduced)..............................................................100 mg
• Glutathione Agarose Resin (50% slurry)..................................1.5 ml

Images:
(Click image to enlarge)

A procedure for capturing K29/K33 polyubiquitinated proteins in cell or tissue lysates

GST-TRABID(NZF1) pulldown assay
1. 20 ug cell lysates
2. GST pulldown
3. GST-TRABID(NZF1) pulldown