Additional Information
Product Name: | K27-linked diUb |
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Also Known As: | N/A |
Catalog No.: | D5210 |
Size: | 25 µg |
Molecular Weight: | 17113 Da by MS |
Species: | Human |
Source: | Synthetic |
Stock: | Product in lyophilized powder form |
Concentration: | N/A |
Quality Assurance: | ≥95% by RP-HPLC and SDS-PAGE |
Storage: | Powder at −20°C; solution at −80°C. Please avoid multiple freeze/thaw cycles. |
PDF Data Sheet: | PDF Datasheet, MSDS |
NCBI RefSeq: | N/A |
Image(s): | (Click image to enlarge) |
Shipping Method: | Room temperature shipping |
References: | 1. A. Faesen et al., (2011) Chemistry & Biology, 18, 1550. 2. I. Dikic et al., (2010) Nature Reviews Molecular Cell Biology 10, 659. 3. J. D. F. Licchesi et al., (2012) Nature Structural & Molecular Biology 19, 62. 4. F. El Oualid et al., (2010) Angewandte Chemie Int. Ed. 49, 10149. |
Details
This product is a native K27-linked diUb which can be used as a substrate of deubiquitinating enzymes. It can also be used to investigatelinkage specificity of proteins that contain ubiquitin-associated domains or ubiquitin-interacting motifs. This product is formed by chemical ligation.
Sample Preparation:
Dissolve the powder in DMSO (e.g. 50 μg in 2.5 μL = 1169 μM) and add this DMSO stock slowly to the required buffer (please note order of addition). General used concentrations vary from 0.1 – 1 μM. Images: (Click image to enlarge)
Sample Preparation:
Dissolve the powder in DMSO (e.g. 50 μg in 2.5 μL = 1169 μM) and add this DMSO stock slowly to the required buffer (please note order of addition). General used concentrations vary from 0.1 – 1 μM. Images: (Click image to enlarge)