Additional Information
Product Name: | Fluorescein labeled K63-Ub(2-7) |
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Also Known As: | Fluorescein K63 Ub(2-7) |
Catalog No.: | D2610 |
Size: | 25 µg |
Molecular Weight: | N/A |
Species: | Human |
Source: | Bacterial recombinant |
Stock: | 20 mM Tris, 150 mM NaCl, 2 mM βME, 10% Glycerol |
Concentration: | See tube label |
Quality Assurance: | ~90% by SDS-PAGE, see datasheet for gel image |
Storage: | Store at -80°C; avoid multiple freeze-thaw cycles |
PDF Data Sheet: | PDF Datasheet, MSDS |
NCBI RefSeq: | N/A |
Image(s): | D2610 image |
Shipping Method: | Dry ice shipping |
References: | No |
Details
This product contains authentic K63-linked isopeotide bonds, synthesized by an enzymatic reaction containing UbE1, GST-E2-25K and fluorescein-Ub (catalog # E1155/E1156). Mainly contain Ub(2-7) chains with residual monoubiquitin. Can be visualized with in-gel Fluorescein fluorescence (excitation at ~490 nm), that is more quantitative and sensitive than immunoblotting in assays including deubiquitination and ubiquitin binding.