Fluorescein labeled K48-Ub(2-10)

This product contains authentic K48-linked isopeotide bonds, synthesized by an enzymatic reaction containing UbE1, GST-E2-25K and fluorescein-Ub (catalog # E1155/E1156). Mainly contain Ub(2-10) chains with residual monoubiquitin. Can be visualized with in-gel Fluorescein fluorescence (excitation at ~490 nm), that is more quantitative than immunoblotting and sensitive in assays including deubiquitination and ubiquitin binding.

Additional Information

Product Name: Fluorescein labeled K48-Ub(2-10)
Also Known As: Fluorescein K48 Ub(2-10)
Catalog No.: D1510
Size: 25 µg
Molecular Weight: N/A
Species: Human
Source: Bacterial recombinant
Stock: 20 mM Tris, 150 mM NaCl, 2 mM βME, 10% Glycerol
Concentration: See tube label
Quality Assurance: ~90% by SDS-PAGE, see datasheet for gel image
Storage: Store at -80°C; avoid multiple freeze-thaw cycles
PDF Data Sheet: PDF DatasheetMSDS
NCBI RefSeq: N/A
Image(s): D1510 image

Shipping Method: Dry ice shipping
References: No

Details

This product contains authentic K48-linked isopeotide bonds, synthesized by an enzymatic reaction containing UbE1, GST-E2-25K and fluorescein-Ub (catalog # E1155/E1156). Mainly contain Ub(2-10) chains with residual monoubiquitin. Can be visualized with in-gel Fluorescein fluorescence (excitation at ~490 nm), that is more quantitative and sensitive than immunoblotting in assays including deubiquitination and ubiquitin binding.