6xHis-Non-cleavable Linear Ub4

6xHis-non-cleavable linear Ub4 contains a 3xHA tag followed by a TEV cleavage site after the 6xHis tag. The G76V substitution was introduced in the first five Ub moieties and the last Ub has G76. The protein was expressed in E. coli and purified.

Additional Information

Product Name: 6xHis-Non-cleavable Linear Ub4
Also Known As: N/A
Catalog No.: D4430
Size: 100 µg
Molecular Weight: N/A
Species: Human
Source: Bacterial recombinant
Stock: 20 mM Tris, 150 mM NaCl, 2 mM βME, 10% Glycerol
Concentration: See tube label
Quality Assurance: ~85% by SDS-PAGE, see datasheet for gel image
Storage: Store at -80°C; avoid multiple freeze-thaw cycles
PDF Data Sheet: PDF DatasheetMSDS
NCBI RefSeq: N/A
Image(s): (Click image to enlarge)

Coomassie-stained SDS-PAGE
Lane 1: Molecular weight markers
Lane 2: 5 µg purified 6xHis-Non-cleavable Linear Ub4
Shipping Method: Dry ice shipping
References: 1. Hershko A,et al. (1980) Proc Natl Acad Sci USA 77(4), 1783-1786.
2. Pickart CM, (1997) FASEB 11(13), 1055-1066.
3. Hershko A, et al. (1998) Ann Rev Biochem 67, 425-479.
4. Jiang X, et al. (2012) Nature Reviews Immunology 12, 35-48.

Details

6xHis-non-cleavable linear Ub4 contains a 3xHA tag followed by a TEV cleavage site after the 6xHis tag. The G76V substitution was introduced in the first five Ub moieties and the last Ub has G76. The protein was expressed in E. coli and purified.

Images:
(Click image to enlarge)

Coomassie-stained SDS-PAGE
Lane 1: Molecular weight markers
Lane 2: 5 µg purified 6xHis-Non-cleavable Linear Ub4