6xHis-Non-cleavable K63 PolyUb Chain (2-4)

6xHis-non-cleavable K63 polyUb chains are made of the 6xHis-Ub(L73P) mutant, this substitution makes the formed polyubiquitin chains to be resistant to the majority of deubiquitinating enzymes.

Additional Information

Product Name: 6xHis-Non-cleavable K63 PolyUb Chain (2-4)
Also Known As: N/A
Catalog No.: D2800
Size: 25 µg
Molecular Weight: N/A
Species: Human
Source: Bacterial recombinant
Stock: 20 mM Tris, 150 mM NaCl, 2 mM βME, 10% Glycerol
Concentration: See tube label
Quality Assurance: ~95% by SDS-PAGE, see datasheet for gel image
Storage: Store at -80°C; avoid multiple freeze-thaw cycles
PDF Data Sheet: PDF DatasheetMSDS
NCBI RefSeq: N/A
Image(s):
Coomassie stained SDS-PAGE
Shipping Method: Dry ice shipping
References: 1. Hershko A,et al. (1980) Proc Natl Acad Sci USA 77(4), 1783-1786.
2. Pickart CM, (1997) FASEB 11(13), 1055-1066.
3. Hershko A, et al. (1998) Ann Rev Biochem 67, 425-479.
4. Jiang X, et al. (2012) Nature Reviews Immunology 12, 35-48.

Details

6xHis-non-cleavable K63 polyUb chains are made of the 6xHis-Ub(L73P) mutant, this substitution makes the formed polyubiquitin chains to be resistant to the majority of deubiquitinating enzymes.