6xHis-linear Ub4

The linear ubiquitin chains are formed by conjugating the N-terminal methionine residue of one ubiquitin with the C-teminal glycine residue of another ubiquitin. This product is expressed in E. coli as a fusion protein.

Additional Information

Product Name: 6xHis-linear Ub4
Also Known As: 6xHis-linear Ub4
Catalog No.: D4210
Size: 100 µg
Molecular Weight: 33.1 kDa
Species: Human
Source: Bacterial recombinant
Stock: 20 mM Tris, 150 mM NaCl, 2 mM βME, 10% Glycerol
Concentration: See tube label
Quality Assurance: ~90% by SDS-PAGE, see datasheet for gel image
Storage: Store at -80°C; avoid multiple freeze-thaw cycles
PDF Data Sheet: PDF DatasheetMSDS
NCBI RefSeq: N/A
Image(s):
Coomassie stained SDS-PAGE
Lane 1: Marker
Lane 2: 6xHis-linear Ub4
Lane 3: 6xHis-linear Ub6
Lane 4: 6xHis-linear Ub8
Shipping Method: Dry ice shipping
References: 1. Hershko A, et al. (1980)Proc Natl Acad Sci USA 77(4), 1783 – 1786.
2. Pickart CM, (1997) FASEB 11(13), 1055 – 1066.
3. Hershko A, et al. (1998) Ann Rev Biochem 67, 425 – 479.
4. Jiang X, et al. (2012) Nature Reviews Immunology 12, 35 – 48.

Details

The linear ubiquitin chains are formed by conjugating the N-terminal methionine residue of one ubiquitin with the C-teminal glycine residue of another ubiquitin. This product is expressed in E. coli as a fusion protein.