PolySUMO Capture & Release Kit

The PolySUMO Capture & Release Kit is designed to rapidly enrich polysumoylated proteins from cell or tissue extracts within 6 hours according to a published method (reference 1) with modifications. This approach uses the SIM domains of RNF4 to capture polysumoylated proteins, which are subsequent released by incubating with a competitive peptide that binds SIM.

Additional Information

Product Name: PolySUMO Capture & Release Kit
Also Known As: N/A
Catalog No.: J4410
Size: 1 Kit
Molecular Weight: N/A
Species: N/A
Source: N/A
Stock: Enzyme stocked in 20 mM Tris, 150 mM NaCl, 2 mM βME, 10% Glycerol
Concentration: See tube label
Quality Assurance: Enzyme >95% by SDS-PAGE
Storage: Store at -80°C; avoid multiple freeze-thaw cycles
PDF Data Sheet: PDF datasheet, MSDS
NCBI RefSeq: N/A
Image(s): No
Shipping Method: Dry ice shipping
References: Bruderer R, et al. Purification and identification of endogenous polySUMO conjugates. (2011) EMBO Rep. 12(2):142-8.

Details

This kit is sufficient for purification of polysumoylated proteins from 100 mg or 20 X 5 mg cell or tissue extracts at a concentration of 5 mg/ml.

Description
The PolySUMO Capture & Release Kit is designed to rapidly enrich polysumoylated proteins from cell or tissue extracts within 6 hours according to a published method (reference 1) with modifications. This approach uses the SIM domains of RNF4 to capture polysumoylated proteins, which are subsequent released by incubating with a competitive peptide that binds SIM.

Components

• GST-4XSIM (4 mg/ml)..............................................1 ml
• GST (4 mg/ml).......................................................1.2 ml
• 2X Elution peptide ............................................ 0.45 ml
• Glutathione Agarose Resin(75% slurry)......... 1.0 ml
• Iodoacetamine (IAA)............................................25 mg
• 20X Cell Lysis Buffer..................................... 2 X 1.0 ml
• 1 M NaCl .......................................................4 X 1.25 ml

Images
(Click image to enlarge)


1. Cell lysates before pulldown
2. Cell lysates after pulldown
3. Elution