K29/K33 Polyubiquitin Chain Capture Kit

This kit is designed for enrichment of cellular proteins conjugated with lysine 29/33-linked polyubiquitin chains using GST-TRABID (ZNF1). Polyubiquitinated proteins bound on GST-TRABID (ZNF1) can be precipi-tated using glutathione resin and eluted by a buffer containing 10 mM glutathione. Enriched polyubiqui-tinated proteins can be assayed by immunoblotting or mass spectrometry.

Additional Information

Product Name: K29/K33 Polyubiquitin Chain Capture Kit
Also Known As: N/A
Catalog No.: J4470
Size: 1 Kit
Molecular Weight: N/A
Species: N/A
Source: N/A
Stock: Enzyme stocked in 20 mM Tris, 150 mM NaCl, 2 mM βME, 10% Glycerol
Concentration: See tube label
Quality Assurance: Enzyme >95% by SDS-PAGE
Storage: Store at -80°C; avoid multiple freeze-thaw cycles
PDF Data Sheet: PDF datasheet
NCBI RefSeq: N/A
Image(s):
Images:
(Click image to enlarge)

A procedure for capturing K29/K33 polyubiquitinated proteins in cell or tissue lysates

GST-TRABID(NZF1) pulldown assay
1. 20 ug cell lysates
2. GST pulldown
3. GST-TRABID(NZF1) pulldown


Shipping Method: Dry ice shipping
References: N/A

Details

Description

This kit is designed for enrichment of cellular proteins conjugated with lysine 29/33-linked polyubiquitin chains using GST-TRABID (ZNF1). Polyubiquitinated proteins bound on GST-TRABID (ZNF1) can be precipi-tated using glutathione resin and eluted by a buffer containing 10 mM glutathione. Enriched polyubiqui-tinated proteins can be assayed by immunoblotting or mass spectrometry.

Components

• GST-TRABID (ZNF1) (2 mg/ml each)......................................1 ml
• GST (4 mg/ml)..........................................................................0.5 ml
• Iodoacetamine (IAA).................................................................25 mg
• Glutathione (reduced)..............................................................100 mg
• Glutathione Agarose Resin (50% slurry)..................................1.5 ml

Images:
(Click image to enlarge)

A procedure for capturing K29/K33 polyubiquitinated proteins in cell or tissue lysates

GST-TRABID(NZF1) pulldown assay
1. 20 ug cell lysates
2. GST pulldown
3. GST-TRABID(NZF1) pulldown