GST-TAB2 (NZF)

GST was fused on the N-terminus of the lysine 63 polyubiquitin chain binding domain of TAB2 encompassing amino acids 627-693. This fusion protein can be used for in vitro GST pulldown assays and for enrichment of cellular proteins conjugated with lysine 63 polyubiquitin chains in whole cell or tissue lysates. GST-TAB2 (NZF) can be precipitated using glutathione resin. After washing, GST-TAB2 (NZF) and its bound proteins can be eluted by a buffer containing 10 mM glutathione.

Additional Information

Product Name: GST-TAB2 (NZF)
Also Known As: CHTD2; MAP3K7IP2
Catalog No.: I1721
Size: 500 µg
Molecular Weight: 7.7 kDa
Species: Human
Source: Bacterial recombinant
Stock: 20 mM Tris, 150 mM NaCl, 2 mM βME, 10% Glycerol
Concentration: See tube label
Quality Assurance: ~80% by SDS-PAGE, see datasheet for gel image
Storage: Store at -80°C; avoid multiple freeze-thaw cycles
PDF Data Sheet: PDF DatasheetMSDS
NCBI RefSeq: NM_015093.4
Image(s): (Click image to enlarge)

Coomassie-stained SDS-PAGE
Lane 1: Molecular weight markers
Lane 2: 5 µg purified GST-TAB2 (NZF)
Shipping Method: Dry ice shipping
References: 1. van Wijk SJ, et al. (2013) Nat Protoc. 8(7):1449-58
2. van Wijk SJ, et al. (2012) Mol Cell. 14;47(5):797-809

Details

GST was fused on the N-terminus of the lysine 63 polyubiquitin chain binding domain of TAB2 encompassing amino acids 627-693. This fusion protein can be used for in vitro GST pulldown GST was fused on the N-terminus of the lysine 63 polyubiquitin chain binding domain of TAB2 encompassing amino acids 627-693. This fusion protein can be used for in vitro GST pulldown assays and for enrichment of cellular proteins conjugated with lysine 63 polyubiquitin chains in whole cell or tissue lysates. GST-TAB2 (NZF) can be precipitated using glutathione resin. After washing, GST-TAB2 (NZF) and its bound proteins can be eluted by a buffer containing 10 mM glutathione.

Images:
(Click image to enlarge)

Coomassie-stained SDS-PAGE
Lane 1: Molecular weight markers
Lane 2: 5 µg purified GST-TAB2(627-693)


Note: Dissolve 10 mM glutathione in a neutral buffer could drop pH to 3 - 4. Use 0.2 M NaOH to adjust pH back to neutral.