GST-S5a (UIM)

GST was fused in the N-terminus of the two UIM domains of S5a, which specifically bind K48-linked polyubiquitin chains. This fusion protein can be used for enrichment of K48-linked polyubiquitinated proteins in cell or tissue lysates, and for in vitro GST pulldown assays.

Additional Information

Product Name: GST-S5a (UIM)
Also Known As: S5a-UIM
Catalog No.: I1620
Size: 100 µg
Molecular Weight: 11.3 kDa
Species: Human
Source: Bacterial recombinant
Stock: 20 mM Tris, 150 mM NaCl, 2 mM βME, 10% Glycerol
Concentration: See tube label
Quality Assurance: ~85% by SDS-PAGE, see datasheet for gel image
Storage: Store at -80°C; avoid multiple freeze-thaw cycles
PDF Data Sheet: PDF DatasheetMSDS
NCBI RefSeq: N/A
Image(s): (Click image to enlarge)

Coomassie-stained SDS-PAGE
Lane 1: Molecular weight markers
Lane 2: 5 µg purified GST-S5a (UIM)
Shipping Method: Dry ice shipping
References: 1. Chiariotti L, et al. (1998) Genomics 47(2), 258 – 265.
2. Fedele M, et al. (2000) J Biol Chem 275(11), 7894 – 7901.
3. Hunter T, Sun H (2008) Ernst Schering Found Sump Proc (1), 1 – 16.
4. Tatham MH, et al. (2008) Nat Cell BIol 10(5), 538 – 546.
5. Bruderer R, et al. (2011) EMBO Rep 12(2), 142 – 148.

Details

GST was fused in the N-terminus of the two UIM domains of S5a, which specifically bind K48-linked polyubiquitin chains. This fusion protein can be used for enrichment of K48-linked polyubiquitinated proteins in cell or tissue lysates, and for in vitro GST pulldown assays.

Images:
(Click image to enlarge)

Coomassie-stained SDS-PAGE
Lane 1: Molecular weight markers
Lane 2: 5 µg purified GST-S5a (UIM)