GST-NEMO (UBAN)

GST was fused on the N-terminus of the linear polyubiquitin chain binding domain of NEMO en-compassing amino acids 183-339. This fusion protein can be used for in vitro GST pulldown as-says and for enrichment of cellular proteins conjugated with linear polyubiquitin chains in whole cell or tissue lysates. GST-NEMO (UBAN) can be precipitated using glutathione resin. After washing, GST-NEMO (UBAN)and its bound proteins can be eluted by a buffer containing 10 mM glutathione.

Additional Information

Product Name: GST-NEMO (UBAN)
Also Known As: IKBKG; IP; IP1; IP2; FIP3; IPD2; NEMO; FIP-3; Fip3p; AMCBX1; ZC2HC9; IKK-gamma
Catalog No.: I1531
Size: 500 µg
Molecular Weight: 18.4 kDa
Species: Human
Source: Bacterial recombinant
Stock: 20 mM Tris, 150 mM NaCl, 2 mM βME, 10% Glycerol
Concentration: See tube label
Quality Assurance: ~90% by SDS-PAGE, see datasheet for gel image
Storage: Store at -80°C; avoid multiple freeze-thaw cycles
PDF Data Sheet: PDF DatasheetMSDS
NCBI RefSeq: NM_001099857
Image(s): (Click image to enlarge)

Coomassie-stained SDS-PAGE
Lane 1: Molecular weight markers
Lane 2: 5 µg purified GST-NEMO (UBAN)
Shipping Method: Dry ice shipping
References: 1. van Wijk SJ, et al. (2013) Nat Protoc. 8(7):1449-58
2. van Wijk SJ, et al. (2012) Mol Cell. 14;47(5):797-809

Details

GST was fused on the N-terminus of the linear polyubiquitin chain binding domain of NEMO en-compassing amino acids 183-339. This fusion protein can be used for in vitro GST pulldown as-says and for enrichment of cellular proteins conjugated with linear polyubiquitin chains in whole cell or tissue lysates. GST-NEMO (UBAN) can be precipitated using glutathione resin. After washing, GST-NEMO (UBAN)and its bound proteins can be eluted by a buffer containing 10 mM glutathione.

Images:
(Click image to enlarge)

Coomassie-stained SDS-PAGE
Lane 1: Molecular weight markers
Lane 2: 5 µg purified GST-NEMO (UBAN)


Note:
1. NEMO(183-339) may also bind long polyubiquitin chains linked via lysine 63.
2. Dissolve 10 mM glutathione in a neutral buffer could drop pH to 3-4. Use 0.2 M NaOH to ad-just pH back to neutral.